|
|||||
|
|
| 分枝杆菌穿梭表达质粒的构建及外源基因在分枝杆菌中的稳定表达 | |
| 引用本文： | 张大军,皇甫永穆,钱明,梁驹卿,李东. 分枝杆菌穿梭表达质粒的构建及外源基因在分枝杆菌中的稳定表达[J]. 华中科技大学学报(医学版), 1995, 0(4) |
| 作者姓名： | 张大军 皇甫永穆 钱明 梁驹卿 李东 |
| 作者单位： | 武汉同济医科大学实验医学研究中心医学分子生物学研究室 |
| 摘 要： | 卡介苗（ＢＣＧ）是全球应用最广泛的疫苗，也是一种能携带多种致病菌保护性抗原表达的疫苗载体。本研究以ｐＵＣ１９为骨架，分别克隆入ＢＣＧ分泌抗原８５－Ｂ的调节和信号肽序列、Ｎｅｏ基因序列（编码卡那霉素抗性）以及分枝杆菌质粒复制基因，构建成分枝杆菌－大肠杆菌穿梭质粒ｐＢＣＧ－８０００。该重组质粒在分枝杆菌（含ＢＣＧ）、大肠杆菌两个系统中均能稳定复制，并能稳定表达卡那霉素抗性。本研究为把ＢＣＧ和其它分枝杆菌发展成为多价疫苗载体打下了基础。 |
| 关 键 词： | 分枝杆菌，穿梭质粒，表达载体，卡介苗，基因表达 |
Construction of E.coli-Mycobacteria Shuttle Expression Vector Plasmid and Stable Expression of Foreign Gene in Mycobacteria |
|
| Zhang Dajun,Huangfu Yongmu,Qian Ming et al. Construction of E.coli-Mycobacteria Shuttle Expression Vector Plasmid and Stable Expression of Foreign Gene in Mycobacteria[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1995, 0(4) | |
| Authors: | Zhang Dajun Huangfu Yongmu Qian Ming et al |
| Abstract: | Bacillus Calmette-Guerin (BCG),a live attenuated bovine tubercle bacillus,is the most widely used vaccine in the world and is also a useful vaccine vehicle for delivering protective antigens of multiple pathogens.In this study the pUC 19 plasmid was used as a backbone and the regulatory and signal sequences of BCG extracellular antigen 85-B,and Neo gene sequence,which encodes kanamycin resistance, and a mycobacterial plasmid origin of replication(Ori M)were progressively cloned into the pUC19 plasmid.The recombinant E.coli-Mycobacteria shuttle expression plasmid pBCG-8000 was constructed. The pBCG8000 plasmid was able to replicate in both E.coli and mycobacteria(including BCG)systems,and confer stable kanamycin resistance upon transformants.The study should facilitate the developments of BCG and other mycobacteria into multivalent vaccine vectors. |
| Keywords: | mycobacterium shuttle plasmid expression vector BCG veccine gene expression |
| 本文献已被 CNKI 等数据库收录！ | |