Dishevelled2和Vangle2蛋白在过量维甲酸致昆明小鼠腭裂发生中的表达变化及意义

目的探讨Dishevelled2和Vangl2蛋白在过量维甲酸致昆明小鼠腭裂发生中的作用。方法采用免疫组织化学及图像分析方法,检测维甲酸致畸小鼠（实验组）和相应对照组小鼠腭板中Dishevelled2和Vangl2蛋白的表达状况。结果Dishevelled2在实验组间充质的表达量明显高于相应对照组,在腭板上皮表达量先明显高于而后又明显低于相应对照组;Vangl2在实验组腭板间充质中的表达量先明显高于而后又明显低于相应对照组。结论Dishevelled2在间充质细胞中表达的上调以及在上皮细胞中先上调又明显下调和Vangl2在腭板间充质细胞中表达的先上调后下调可能与维甲酸致昆明小鼠腭裂发生相关。

Effects of Dishevelled2 and Vangl2 on the development of cleft palate induced by excess retinoid acid in KM mice

To explore the effects of Dishevelled2 and Vangl2 protein on cleft palate induced by excess retinoid acid in KM mice. MethodsIn both malformed embryos induced by excess retinoid acid (the experimental group) and normal KM mice (the control group),  expressions of Dishevelled2 and Vangl2 in the epithelium of the palatal shelves and the mesenchyma were determined by  immunohistochemistry and image analysis. ResultsExpression of Dishevelled2 in the mesenchyma of the experimental group was significantly higher than that in the control group, however that in the epithelium of the palatal  shelves of the experimental group was significantly higher, but then lower than that in the control group. So was expression of Vanlg2 in the mesenchyma. ConclusionExcess retinoid acid may interfere with normal expression of Dishevelled2 and Vangle protein in developing palates and causes cleft palate in KM mice.