| 高效液相色谱法测定川葛颗粒中葛根素的含量 |
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| 引用本文： | 刘文斌,阎琪. 高效液相色谱法测定川葛颗粒中葛根素的含量[J]. 中国卫生工程学, 2011, 0(1): 71-72 |
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| 作者姓名： | 刘文斌 阎琪 |
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| 作者单位： | [1]吉林省药物研究所,吉林长春130021 [2]长春中医药大学,吉林长春130021 |
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| 摘 要： | 目的建立高效液相色谱法测定川葛颗粒中葛根素含量。方法色谱柱Zorbax Eclipes XDB（150 mm×4.6 mm,5μm）;流动相甲醇∶水（25∶75）;流速1.0 ml/min;检测波长250 nm;柱温25℃。结果葛根素在0.090 4～0.904 0μg范围内线性关系良好（r=0.999 9,n=5）;平均回收率为97.48%（n=9）,RSD为1.47%。结论本方法操作简单、准确可靠,可用于川葛颗粒的质量控制。
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| 关 键 词： | 葛根素 川葛颗粒 高效液相色谱法 |
Determination of Puerarin in Chuange Granules by High Performance Liquid Chromatography |
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| LIU Wen-bin,YAN Qi. Determination of Puerarin in Chuange Granules by High Performance Liquid Chromatography[J]. Chinese Journal of Public Health Engineering, 2011, 0(1): 71-72 |
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| Authors: | LIU Wen-bin YAN Qi |
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| Affiliation: | .(Jilin Institute of Drug,Changchun Jilin 130021,China ) |
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| Abstract: | Objective To establish a high performance liquid chromatography（HPLC） method for determination of puerarin in Chuange granules. Methods Separation of samples was achieved on a Zorbax Eclipes XDB column（150 mm × 4.6 mm,5 μm）.The mobile phase consisted of methanol-water（25：75） at a flow rate of 1.0 ml/min.The detection wavelength was 250 nm and the column temperature was 25℃. Results The standard curves for puerarin were linear in the ranges of 0.090 4-0.904 0 μg（r =0.999 9,n =5）;The average recovery rate was 97.48%（n =9）,RSD was 1.47%. Conclusion The method is fast,simple,specific,reliable,and can be used for quality control of Chuange granules. |
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| Keywords: | Puerarin Chuange granules High performance liquid chromatography |
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