3种品系大鼠对灯盏花素所致 被动皮肤过敏差异性研究

目的:比较F344,SD,Wistar 3种品系大鼠对灯盏花素诱导的被动皮肤过敏(passive cutaneous anaphylaxis,PCA)反应的差异性。方法:取3种品系大鼠随机分为阴性对照组、天花粉组、灯盏花素组。采用sc给药方式,分别给予各组动物不同的药物,进行大鼠同种或大鼠-小鼠异种PCA试验,检测给予灯盏花素后动物皮肤蓝斑吸光度(A),比较药物对F344,SD,Wistar 3种品系大鼠的A变化率。结果:大鼠同种PCA试验显示:3种品系大鼠注射灯盏花素25 mg.kg-1致敏后,F344大鼠皮肤蓝斑A为0.064±0.005 7,明显升高(P<0.05),SD,Wistar大鼠PCA反应均为阴性。大鼠-小鼠异种PCA试验显示:注射F344大鼠抗血清后,小鼠异种PCA反应为阳性,A为0.10±0.01(P<0.05),注射SD,Wistar大鼠抗血清后,反应均为阴性。结论:2种试验方法结果均显示,3种品系大鼠对灯盏花素的敏感性存在差异,其中以F344大鼠最为敏感。

Differences in Sensitivity of Passive Cutaneous Anaphylaxis Induced by Breviscapine Among Three Rat Strains

Objective: To compare the differences in sensitivity of passive cutaneous anaphylaxis (PCA) caused by breviscapine among Sprague Dawley (SD), Wistar, and Fischer344(F344) rats. Method: The rats in the 3 strains were randomly divided into negative control group, trichosanthin group and breviscapine group. The homologous PCA experiment was performed by using three different strains of rat and xenogenic PCA by using rats and mice. Breviscapine was given as allergen. The absorbance was measured to discriminate the severity of PCA. Result: The rat homologous PCA revealed that after injection with 25 mg · kg^-1 breviscapine, F344 rat showed positive reaction, the absorbance was 0.064±0.005 7 and increased significantly (P<0.05), but SD rat and Wistar rat showed negative reaction. Xenogeneic PCA showed that the mice injected breviscapine antiserum of F344 rat, demonstrated a positive reaction (P<0.05),the absorbance was 0.10±0.01,the others received breviscapine antiserum of SD or Wistar rats showed negative reactions. Conclusion: There are differences in sensitivity to breviscapine among three rat strains, and F344 rat is the most sensitive one.