Induction of growth arrest in colorectal cancer cells by cold plasma and gold nanoparticles |
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| Authors: | Shiva Irani Zhohreh Shahmirani Seyed Mohammad Atyabi Shahriar Mirpoor |
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| Affiliation: | 1.Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran;2.Department of Pilot Biotechnology, Pasteur Institute of Iran, Tehran, Iran;3.Department of Plasma, Shahid Beheshti University, Tehran, Iran |
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| Abstract: | IntroductionGuided treatments with nanoparticles and cold atmospheric plasma are a new approach in cancer therapy. Plasma is an ionized gas that has reactive and energetic particles and can be produced in the laboratory by different methods.Material and methodsPlasma jet therapy was employed to irradiate HCT-116 cells (human colorectal cancer cells) which were cultured in the presence of gold nanoparticles (GNPs). Cell cytotoxicity was tested with 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), and cancerous cell apoptosis was shown by 4’,6-diamidino-2-phenylindole (DAPI) staining.ResultsThe results showed that cell death was increased significantly with p < 0.001 by cold atmospheric plasma in the presence of gold nanoparticles.ConclusionsIt appears that non-thermal plasma and gold nanoparticles synergism is a promising approach in colon cancer therapy. |
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| Keywords: | nanoparticles non-thermal plasma colorectal cancer |
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