| 高效液相色谱法测定消痰咳片的含量 |
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| 引用本文： | 王红梅,徐丽美,张水龙. 高效液相色谱法测定消痰咳片的含量[J]. 中国现代应用药学, 2002, 19(3): 220-222 |
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| 作者姓名： | 王红梅 徐丽美 张水龙 |
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| 作者单位： | 厦门市药品检验所 厦门361012(王红梅，徐丽美)，厦门市药品检验所 厦门361012(张水龙) |
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| 摘 要： | 目的：采用反相色谱法同时测定消痰咳片中两种主要成分甲氧苄啶和磺胺体的含量。方法：以乙腈：0.1%H3PO4(15:85)为流动相，检测波长254nm，HPLC法测定含量。结果：试验表明，甲氧苄啶和磺胺林在0.8-8ug范围内呈良好的线性关系，回归方程分别为Y＝－1127.1＋110.2x(r=0.9994),Y=-1852.3 256.2x(r=0.9996),相对标准偏差分别为2.1%和0.8%。结论：该方法简便、准确、可靠。
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| 关 键 词： | 高效液相色谱法 消痰咳片 甲氧苄啶 磺胺林 含量测定 |
Simultaneous Determination of Trimethoprim and Sulfalene in Xiao Tang Ke Tablets by High performance Liquid Chromatography |
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| Wang HongMei (Wang HM,Xu Li Mei(Xu LM,Zhang Shui Long(Zhang SL. Simultaneous Determination of Trimethoprim and Sulfalene in Xiao Tang Ke Tablets by High performance Liquid Chromatography[J]. The Chinese Journal of Modern Applied Pharmacy, 2002, 19(3): 220-222 |
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| Authors: | Wang HongMei (Wang HM Xu Li Mei(Xu LM Zhang Shui Long(Zhang SL |
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| Abstract: | OBJECTIVE:To develop a reversed phase high performance liquid chromatographic method for the simultaneous determination of Trimethoprim and Sulfalene in Xiao Tang Ke Tablets. METHOD:The mobile phase was acetonitrile 15% and H3PO4(0.1%) 85%.The detector wavelength was set at 254nm and the flow rate was 2.0ml/min.RESULTS:The linear ranges were 0.8 8ug/ml(Trimethopim)(Y=-1127.1+110.2x, r=0.9994) and Sulfalene (Y=-1852.3+256.2x,r=0.9996). The relative standard deviations were 2.1%(Trimethoprim) and 0.8%(Sulfalene) ( n =5).CONCLUSION:This method was simple,rapid and sensitive. |
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| Keywords: | reversed phase highperformance liquid chromatographic Xiao Tang Ke Tablets trimethoprim sulfalene |
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