栎树桑黄提取物诱导胃癌细胞凋亡作用的研究

目的 研究栎树桑黄乙醇提取物(OPL)对人胃癌细胞SGC-7901的抗肿瘤和诱导凋亡作用.方法 用不同浓度的OPL(0、40、80、160、240、320、400μg/ml)作用于4种不同人癌SGC-7901、HCT-116、MCF-7、Hela细胞48 h,MTT法检测药物抑制细胞增殖率;流式细胞术检测OPL对SGC-7901细胞周期的影响,Hoechst染色观察OPL对细胞核形态变化影响,Annexin-V/PI双染流式细胞仪检测OPL对细胞凋亡的影响,Western blot法检测SGC-7901周期蛋白Cyclind1的表达,凋亡相关蛋白Bcl-2、Bax、cleaved PARP蛋白的表达.结果 OPL明显呈现浓度依赖性地抑制SGC-7901细胞增殖,诱导细胞凋亡,将细胞周期阻滞在G0/G1期(P＜0.05).Western blot法显示OPL上调Bax、cleaved PARP蛋白表达,下调Bcl-2、Cyclind1蛋白的表达(P＜0.05).结论 OPL呈现浓度依赖性诱导胃癌SGC-7901细胞凋亡,并将细胞周期阻滞在G0/G1期.

Study on the apoptosis of gastric cancer cells induced by extractions from oak Phellinus linteus

Objective To investigate the antitumor and apoptosis induction effect of extractons from oak Phellinus linteus(OPL) against human gastric SGC-7901 cancer.Methods Human SGC-7901,HCT-116,MCF-7,Hela cancer cells were cultured with OPL(0,40,80,160,240,320,400 μg/ml) for 48 h.The proliferation was evaluated via MTT assays,the cell cycle was analyzed through flow cytometry,Hoechst staining was used to observe OPL impact on nuclear shape change,and the apoptosis state was analyzed by Annexin-V/PI staining assays.The expression of apoptosis-related and cycle-related proteins was determined by Western blot.Results OPL could significantly reduce cell viability,induce apoptosis,and arrest SGC-7901 cell cycle at the G0/G1 phases(P ＜0.05).Western blot method further confirmed that OPL could increased bax and the subsequent cleavage of poly (ADP-ribose) polymerase,decreased CyclinD1 and Bcl-2 protein expression (P ＜ 0.05).Conclusion Our findings suggest that OPL can induce apoptosis in human gastric SGC-7901 cancer cells,and cause cell cycle arrest in G0/G1 phase.