甲状腺乳头状癌及相关病变Jab1、Skp2和p27蛋白的表达及意义

目的 探讨细胞周期有关的因子c-Jun激活区域结合蛋白-1(Jab1)、S期激酶相关蛋白2(Skp2)和细胞周期素依赖激酶抑制剂p27蛋白在结节性甲状腺肿、桥本甲状腺炎和甲状腺乳头状癌中的表达及意义.方法 采用免疫组织化学方法检测结节性甲状腺肿、桥本甲状腺炎、伴结节性甲状腺肿的甲状腺乳头状癌和伴桥本甲状腺炎的甲状腺乳头状癌中Jab1、Skp2和p27蛋白的表达.结果 Jab1、Skp2蛋白的阳性表达率在伴结节性甲状腺肿的甲状腺乳头状癌者(86.9℅、82.6℅)和伴桥本甲状腺炎的甲状腺乳头状癌者(83.3℅、66.7℅)均分别明显高于结节性甲状腺肿者(26.3℅、0℅)和桥本甲状腺炎者(23.8℅、4.8℅)(P<0.01).p27蛋白的阳性表达率在伴结节性甲状腺肿的甲状腺乳头状癌者(39.1℅)和伴桥本甲状腺炎的甲状腺乳头状癌者(45.6℅)均分别明显低于结节性甲状腺肿者(89.5℅)和桥本甲状腺炎者(100℅)(P<0.01).甲状腺乳头状癌中,癌组织?>1 cm者Jab1蛋白阳性表达率明显高于癌组织?≤1 cm者(P<0.01);Jab1和Skp2蛋白表达与p27蛋白表达均呈负相关性(rs=-0.344,P=0.028;rs=-0.421,P=0.006);Jab1蛋白表达与Skp2蛋白表达呈正相关性(rs=0.43,P=0.005);肿瘤侵袭区域Jab1蛋白表达阳性的细胞百分率(61.22±8.99)℅明显高于肿瘤中心区域(42.61±9.46)℅(P<0.01).结论 Jab1、Skp2和p27蛋白表达可能参与结节性甲状腺肿和桥本甲状腺炎癌变为甲状腺乳头状癌的过程,甲状腺乳头状癌中Jab1、Skp2和p27蛋白表达之间可能存在相互调节的关系,Jab1蛋白表达可能与甲状腺乳头状癌细胞的迁移和侵袭有关.

Expression and significance of Jab1 , Skp2 and p27 in papillary thyroid carcinoma and its related lesion

Objective To investigate the expression and significance of cell cycle related factors Jun activation-do-main binding protein 1 ( Jab1 ) , S kinase protein 2 ( Skp2 ) and cyclin-dependent kinases inhibitor p27 in nodular goiter, Hashimoto's thyroiditis and papillary thyroid carcinoma. Methods The expressions of Jab1, Skp2 and p27 protein were examined by immunohistochemistry method in 19 cases of nodular goiter, 21 cases of Hashimoto' s thy-roiditis, 23 cases of papillary thyroid carcinoma with nodular goiter and 18 cases of papillary thyroid carcinoma with Hashimoto's thyroiditis. Results The positive rates of Jab1 and Skp2 protein expression, thyroid papillary carcino-ma with nodular goiter ( 86. 9℅, 82. 6℅) and with Hashimoto ' s thyroiditis ( 83. 3℅, 66. 7℅) were significnatly higher than nodular goiter(26. 3℅,0℅)or Hashimoto's thyroiditis(23. 8℅,4. 8℅)(P<0. 01), respectively. The positive rate of p27 protein expression, thyroid papillary carcinoma with nodular goiter(39. 1℅) and with Hashimo-to's thyroiditis(45. 6℅) were significnatly lower than nodular goiter(89. 5℅) and Hashimoto's thyroiditis(100℅) (P<0. 01),respectively. In thyroid papillary carcinoma, the positive rate of Jab1 protein expression, the thyroid papillary carcinoma with diameter greater than 1 cm were higher than the thyroid papillary carcinoma with diameter less than 1 cm ( P <0. 01 );Jab1 and Skp2 expressions were negatively correlated with p27 expression ( rs =-0. 344, P=0. 028;rs= -0. 421, P=0. 006); Jab1 expression was positively correlated with Skp2 expression ( rs=0. 43, P=0. 005);the percent rate of carcinoma cells with Jab1 expression was significantly higher in the in-vasive region(61. 22 ±8. 99)℅ compared to the center of the tumors(42. 61 ±9. 46)℅(P<0. 01). Conclusion Jab1, Skp2 and p27 protein may have participated in canceration of nodular goiter and Hashimoto's thyroiditis. In papillary thyroid carcinoma, Jab1,Skp2 and p27 may have mutually positive regulation. Jab1 protein expression may be associated with increased cell migration and invasion in papillary thyroid carcinoma.