| CCL21对胃癌SGC-7901细胞发生上皮间质转化的影响 |
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| 引用本文： | 史冠男,袁媛,彭琼,戴夫.CCL21对胃癌SGC-7901细胞发生上皮间质转化的影响[J].安徽医药,2017,21(1):113-115. |
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| 作者姓名： | 史冠男 袁媛 彭琼 戴夫 |
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| 作者单位： | 合肥市第一人民医院消化内科,安徽合肥,230061;合肥市滨湖医院中心实验室,安徽合肥,230051 |
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| 基金项目： | 安徽省合肥市重点科技项目 |
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| 摘 要： | 目的 研究CCL21能否参与诱导胃癌SGC-7901细胞发生上皮间质转化.方法 取不同浓度(0、50、100、150μg·L-1)的CCL21作用胃癌SGC-7901细胞,划痕试验检测该细胞侵袭能力.镜下观察该细胞形态的变化.Western blot分别检测E-cadherin、N-cadherin、Vimentin、MMP-9的蛋白表达.结果 150μg·L-1浓度的CCL21作用胃癌SGC-7901细胞后,较其它浓度侵袭能力强,同时使胃癌SGC-7901细胞的胞体逐渐变为长梭状,部分可见伪足生成.E-cadherin蛋白表达下降(P<0.05),N-cadherin、Vimentin、MMP-9蛋白表达升高(P<0.05).结论 CCL21可能参与诱导胃癌SGC-7901细胞发生上皮间质转化.
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| 关 键 词： | 上皮间质转化 趋化因子 胃癌 |
| 收稿时间： | 2016/8/10 0:00:00 |
| 修稿时间： | 2016/9/17 0:00:00 |
Effects of CCL21 on inducing epithelial-mesenchymal transition in gastric cancer cells SGC-7901 |
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| SHI Guannan,YUAN Yuan,PENG Qiong and DAI Fu.Effects of CCL21 on inducing epithelial-mesenchymal transition in gastric cancer cells SGC-7901[J].Anhui Medical and Pharmaceutical Journal,2017,21(1):113-115. |
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| Authors: | SHI Guannan YUAN Yuan PENG Qiong and DAI Fu |
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| Institution: | Department of Gastroenterology,The First People''s Hospital of Hefei,Hefei,Anhui 230061,China,The Central Laboratory in Binhu Hospital,Hefei,Anhui 230051,China,Department of Gastroenterology,The First People''s Hospital of Hefei,Hefei,Anhui 230061,China and Department of Gastroenterology,The First People''s Hospital of Hefei,Hefei,Anhui 230061,China |
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| Abstract: | Objective To investigate whether CCL21 can induce epithelial-mesenchymal transition in gastric cancer cells SGC-7901. Methods SGC-7901 cells were induced by CCL21 in different concentration(0,50,100,150μg·L-1)and then the ability of inva-sion and metastasis was detected by scratch testing.Morphological changes in forms were observed.The protein expressions of E-cadher-in,N-cadherin,Vimentin,MMP-9 were detected by Western blot.Results After being induced by CCL21 in 150μg·L-1,SGC-7901 cells achieved faster ability of invasion and metastasis.SGC-7901 cells formed into a long spindle and pseudopod could be seen.The ex-pressions of N-cadherin,Vimentin,and MMP-9 rose(P<0.05)while that of E-cadherin obviously descended(P<0.05).Conclusions CCL21 may induce epithelial-mesenchymal transition in gastric cancer cell and promote the invasion and metastasis. |
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| Keywords: | Epithelial-mesenchymal transition Chemokine Gastric cancer |
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