1年生黄芪中黄芪多糖对小鼠免疫器官指数的影响

目的]探讨一年生黄芪中黄芪多糖对小鼠免疫器官指数的影响.方法]取1年生黄芪经水提取后,用质量浓度为500,600,800mL/L乙醇分别进行沉淀,获得相应乙醇沉淀提取的黄芪多糖.采用硫酸-苯酚法分别测定各质量浓度乙醇提取的黄芪多糖中多糖含量,每日分别腹腔注射给予小鼠200mg/kg不同质量浓度乙醇提取的黄芪多糖10d,实验第7日开始每日分别腹腔注射给予各组小鼠环磷酰胺80mg/kg,连续4d,处死小鼠,测定脾脏及胸腺指数.结果]500,600,800mL/L乙醇提取的黄芪多糖中多糖含量分别为530.7,407.6,463.8g/kg.500,600,800mL/L乙醇提取的黄芪多糖组小鼠脾脏指数均显著高于环磷酰胺组;500mL/L乙醇提取黄芪多糖组小鼠胸腺指数显著高于环磷酰胺组.结论]1年生黄芪中的黄芪多糖对环磷酰胺所致小鼠免疫功能低下有拮抗作用.

Effects of polysaccharides from the annual Astragalus on immuno-organs coefficients on mice

OBJECTIVE To evaluate effects of polysaccharides from the annual Astragalus on immuno-organs coefficients on mice.METHODS The polysaccharides was precipitated with 500,600 and 800 mL/L ethanol respectively after the water extraction;the content of polysaccharides was determined by Anthrone Colorimetry.The mice were administered with the polysaccharides of 500,600 and 800 mL/L ethanol respectively by ip for 10 days(200 mg/kg).From the 7th day,the mice were administered with the cyclophosphamide by ip(80 mg/kg)for 4 days,and then the weights of spleen and thymus were measured.RESULTS The content of polysaccharides in the 500,600 and 800 mL/L ethanol groups was 530.7,407.6 and 463.8 g/kg,respectively.The mice spleen index of the three ethanol groups increased significantly than the CY group.And only the thymic index of 500 mL/L ethanol group increased significantly than the CY group.CONCLUSION The polysaccharides in the annual Astragalus have the inhibitory effects to the immunosuppressed mice induced by cyclophosphamide.