| 抗弓形虫IgM抗体时间分辨荧光免疫分析法的建立及初步应用 |
| |
| 引用本文： | 周彬,潘宇红,黄飚,黄旋,王柯,朱岚,张珏. 抗弓形虫IgM抗体时间分辨荧光免疫分析法的建立及初步应用[J]. 上海医学检验杂志, 2010, 0(4) |
| |
| 作者姓名： | 周彬 潘宇红 黄飚 黄旋 王柯 朱岚 张珏 |
| |
| 作者单位： | 江苏省原子医学研究所卫生部核医学重点实验室;江苏省无锡市第三人民医院检验科; |
| |
| 摘 要： | 目的采用时间分辨荧光免疫分析法(TRFIA)建立高灵敏的抗弓形虫(TOX)IgM抗体的检测方法。方法采用二抗间接法建立抗TOX IgM抗体的TRFIA。结果方法的批内和批间变异系数(CV)分别为1.9%和3.8%,敏感性为0.4 U/mL,可测范围为0.4~200 U/mL,20%、50%和80%的有效剂量(ED20、ED50和ED80)分别为36.3、88.2和147.8 U/mL。检测健康体检人员的血清,标本的测定值为(5.3±2.3)U/mL,建议阳性阈值为10 U/mL。结论抗TOX IgM抗体的TRFIA敏感性高、稳定性好,在优生优育及器官移植上具有很好的应用前景。
|
| 关 键 词： | 弓形虫 IgM抗体 时间分辨荧光免疫分析法 |
Establishment and clinical application of the time-resolved fluoroimmunoassay of anti-Toxoplasma-IgM antibody |
| |
| Abstract: | Objective To develop a time-resolved fluoroimmunoassay(TRFIA) with high sensitivity for the detection of anti-Toxoplasma(TOX)-IgM antibody.Methods The tested specimen or reference standard was added to micro-titer plate coated with the TOX antigen and the europium-labelled anti human IgM antibody was added.Results The between-run and within-run coefficients of variation(CV) of this TRFIA were 1.9% and 3.8% respectively.The sensitivity was 0.4 U/mL.The range of testing was from 0.4 to 200 U/mL.The TRFIA prov... |
| |
| Keywords: | Toxoplasma IgM antibody Time-resolved fluoroimmunoassay |
| 本文献已被 CNKI 等数据库收录！ |
|
