p53基因第72密码子多态性与青海地区胃癌易感性

目的 探讨p53基因第72密码子（Arg72Pro）单核苷酸多态性（SNP）与青海地区胃癌及其胃癌发生部位易感性的关系.方法 采用TaqMan实时荧光定量PCR方法,检测胃癌患者及健康对照者外周血标本p53基因Arg72Pro多态性.结果 p53基因Arg72Pro 3种基因型Arg/Arg、Pro/Pro、Arg/Pro在胃癌组中的频率分别为21.86%、25.00%、53.14%,在对照组中分别为16.67%、31.94%、51.39%.等位基因Arg和等位基因Pro在胃癌中的频率分别为48.44%、51.56%,在对照组中分别为42.36%、57.64%.两组间基因型频率及等位基因频率分布均无统计学意义（P分别为0.59、0.32）,p53基因型分布和胃癌发生的部位亦不具有相关性（P=0.74）.结论 p53基因Arg72Pro多态性与青海地区胃癌易感性无明显相关,与胃癌发生部位也无明显相关性.

THE POLYMORPHISMS OF NUMBER 72 CODEN OF p53 GENE WITH SUSCEPTIBILITY OF GASTRIC CANCER IN QINGHAI AREA

Objective To explore the correlation between genetic polymorphism of p53 codon 72 and susceptibility of gastric cancer in Qinghai area.Methods The Arg/Pro polymorphism of p53 gene was detected by real-time PCR in 64 patients with gastric cancer and 72 healthy controls.Results The proportions of p53 codon 72 gene type Arg/Arg,Arg/Pro and Pro/ Pro were 21.86%,25.00%,53.14% in gastric cancer group,and 16.67%,31.94%,51.39% in control group,respectively.Arg and Pro alleles in gastric cancer group were 48.44%,51.56%,while in the control group were 42.36%,57.64%,respectively.There was no significant difference between gastric cancer group and the control group in genotype and allele distribution frequency（P=0.59,P=0.32）.There was no relationship between the distribution of p53 gene and the site of gastric cancer（P=0.74）.Conclusion Polymorphism at codon 72 of p53 gene may be not correlated with gastric cancer in Qinghai area, and also no correlation with the site of gastric cancer.