雪灵芝水提取物对人胃腺癌MGC-803细胞Bcl-2，Bax基因表达的影响

目的研究雪灵芝水提取物对人胃腺癌MGC-803细胞Bcl-2和Bax基因表达的影响。方法不同浓度的雪灵芝水提取物处理体外培养的MGC-803细胞，采用四甲基偶氮噻唑蓝（MYr）法检测细胞存活水平；应用流式细胞术检测细胞周期和细胞凋亡水平；采用逆转录聚合酶链反应（RT—PCR）和蛋白印迹法（Western Blot）测定人胃腺癌MGC-803细胞的Bcl-2和Bax基因表达情况。结果雪灵芝水提取物作用于人胃腺癌MGC～803细胞后，0．1～0．8mg／mL浓度组细胞存活水平随药物浓度的增大而减小（P〈0．05）；0．8mg／mL浓度组MGC-803细胞G．期比例为（61．23±6．45）％、S期细胞比例为（26．86±3．38）％、细胞凋亡率为（0．07±0，01）％，与对照组相比，P〈0．05；与对照组相比，0．8mg／mL浓度组Bcl-2 mRNA表达降低、Bax mRNA表达增加（P〈0．05）；0．2～0．8mg／mL浓度组Bcl-2蛋白表达降低、Bax蛋白表达增加。结论雪灵芝水提取物在一定浓度范围内可降低MGC-803细胞的存活水平，使细胞发生凋亡，其机制可能为通过下调Bcl-2基因、上调Bax基因表达，促进细胞凋亡发生。

INFLUENCE OF ARENARIA KANSUENSIS AQUEOUS EXTRACT ON EXPRESSIONS OF BCL- 2 AND BAX GENE IN HUMAN STOMACH CARCINOMA CELL LINE MGC- 803

Objective To study the effect of Arenaria Kansuensis Aqueous Extract （AKAE） on the expressions of Bcl - 2 and Bax gene in human gastric cancer cell line MGC - 803. Methods MGC - 803 cells were incubated with AKAE at different concentration , cellular survival level was tested by MTT;Cell cycle and apoptosis were detected by flow cytometer （FCM） ; RT - PCR and Western Blot were used to detect the gene expression level of the Bcl - 2 and Bax. Results After treatment with AKAE, MGC - 803 cellular survival level gradually decreased at dose of 0.1 - 0.8mg/mL （ P 〈 0.05 ） ; The percentage of MGC - 803 G1 phase was （61.23 ± 6.45 ） %, S phase was （26.86 ± 3.38） % and the apoptotic level was （0.07 ± 0.01 ） % at dose of 0 . 8mg/mL, compared with control group（ P 〈 0.05 ） ;At the dose of 0.8mg/mL, the Bcl- 2 mRNA expression decreased and the Bax mRNA expression increased, respectively compared with control group（P 〈 0. 05） ;At the dose of 0. 2 -0.8mg/mL, protein expression of Bcl -2 was decreased while protein expression of Bax was increased. Conclusion AKAE can decrease the Bcl -2 mRNA and protein expression and increase the Bax mRNA and protein expression in MGC -803 cells, so that it plays a role in inducing cell apoptosis.