|
|||||
|
|
| 亚慢性氟中毒对大鼠骨组织Runx2 mRNA表达的影响 | |
| 引用本文： | 郭晓英,吴思思,何杨,孙贵范. 亚慢性氟中毒对大鼠骨组织Runx2 mRNA表达的影响[J]. 中国工业医学杂志, 2011, 24(2): 86-88+161 |
| 作者姓名： | 郭晓英 吴思思 何杨 孙贵范 |
| 作者单位： | 中国医科大学公共卫生学院地球化学性疾病研究室辽宁省砷生物学作用与砷中毒重点实验室; |
| 基金项目： | 国家自然科学基金资助项目(No.30600509):氟对破骨细胞分化传导环路的影响 |
| 摘 要： | 目的观察亚慢性氟中毒对大鼠骨组织中转录因子Runx2 mRNA表达的影响,探讨Runx2在氟骨症发生中的可能作用。方法 40只Wistar大鼠按体重随机分成4组,染氟组大鼠饮用含氟50、100、150 mg/L的自来水,对照组大鼠饮用自来水;3个月后测定大鼠血清及骨组织氟含量,并采用SYBR Green Real-time RT-PCR法检测骨组织中Runx2 mRNA表达情况,同时观察骨组织病理学改变。结果 HE染色显示染氟组大鼠呈现骨皮质增厚、骨小梁增多等骨硬化表现;染氟组大鼠骨组织中Runx2 mRNA表达显著高于对照组,且随染毒剂量升高,表达亦逐渐升高。结论过量的氟可能通过增加骨组织Runx2基因表达水平来促进间充质干细胞向成骨细胞系分化,使骨形成增加。 |
| 关 键 词： | 氟中毒 转录因子Runx2 实时定量反转录聚合酶连锁反应(Real-time RT-PCR) |
Effects of subchronic fluorosis on expre ssion of Runx2 mRNA in bone tissue of rat |
|
| GUO Xiao-ying,WU Si-si,HE Yang,SUN Gui-fan. Effects of subchronic fluorosis on expre ssion of Runx2 mRNA in bone tissue of rat[J]. Chinese Journal of Industrial Medicine, 2011, 24(2): 86-88+161 | |
| Authors: | GUO Xiao-ying WU Si-si HE Yang SUN Gui-fan |
| Affiliation: | GUO Xiao-ying,WU Si-si,HE Yang,SUN Gui-fan(Department of Biogeochemical Disease,Liaoning Provincial Key Laboratory of Arsenic Biological Effect and Poisoning,School of Public Health,China Medical University,Shenyang 110001,China) |
| Abstract: | Objective Observing the effect of subchronic fluoride poisoning on the expression of Runx2 mRNA in bone tissue of rats,to explore the role of Runx2 played in pathogenesis of skeletal fluorosis.Methods 40 Wistar rats were divided into four groups randomly according to body weight.Rats in experimental groups were given 50,100 or 150 mg/L of fluorine containing water,the controls only given tap water.Three months later,the fluorine levels in serum and bone tissue and the expression of Runx2 mRNA in bone tissue... |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《中国工业医学杂志》浏览原始摘要信息 | |
| 点击此处可从《中国工业医学杂志》下载免费的PDF全文 | |