反相高效液相色谱法测定大蒜素的血药浓度

目的：建立反相高效液相色谱法测定血清中大蒜素的浓度。方法：血清样品用１０％三氯醋酸沉淀蛋白，正己烷提取后进样。采用Ｐｈｅｎｏｍｅｎｅｘ Ｃ１８（２５０ｍｍ × ４．６ｍｍ，５μｍ）柱；流动相：乙腈－１％冰醋酸（６２：３８，Ｖ／Ｖ），ｐＨ＝３．５；流速：１．２ｍｌ／ｍｉｎ，在室温下；波长：２４０ｎｍ检测。结果：该方法回收率为（９９．２±６．６）％，最低检测浓度为０．１８μｇ／ｍｌ，大蒜素血药浓度在０．２９～１２．４μｇ／ｍｌ范围内峰面积与浓度线性关系良好（ｒ＝０．９ ９７７），日内 ＲＳＤ＜５．５％（ｎ＝５），日间 ＲＳＤ＜５．８％（ｎ＝５） 结论：本法快速、准确、灵敏，能较好满足大蒜素临床药代动力学研究的需要。

Determination of Allitridum in Human Serum by RP - HPLC

OBJECTIVE: To develope a reversed-phase high performance liquid chromatographic(RP-HPLC) method for the determination of allitridum in human serum.METHODS: The serum sample was injected after being deproteinized with trichloroaceticacid and being extracted with hexane.A Phenomenex C1s was used as the stationary phase and the solution being composed of acetonitrile- 1%HAc(62: 38,V/V)as the mobile phase with a flow rate of 1.2 ml/min .The serum samples were monitored at 240nm.RESULTS: The peak area of allitridum against allitridum concentration was linear with a correlation coefficient of 0.9 977 over the range of 0.29～12.4 μg/ml, the limitation of detection was 0.18 μg/ml.The average recovery was (99.2 ± 6.6) %, within - day RSD＜ 5.5% (n = 5), between - day RSD＜ 5.8% (n = 5) .CONCLUSIONS: This method is rapid, accurate and sensitive,it is very suitable for the investigation on clinical pharmacokinetics of allitridum.