| HPLC测定半夏白术天麻汤中天麻素和鸟苷的含量 |
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| 引用本文： | 王勋,陆家凤,罗珊珊,王现珍,蒋嘉烨,可燕.HPLC测定半夏白术天麻汤中天麻素和鸟苷的含量[J].中国实验方剂学杂志,2010,16(17):44-47. |
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| 作者姓名： | 王勋 陆家凤 罗珊珊 王现珍 蒋嘉烨 可燕 |
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| 作者单位： | 上海中医药大学教学试验中心,上海,201203 |
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| 基金项目： | 国家科技部十一五支撑计划 |
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| 摘 要： | 目的:建立同时测定半夏白术天麻汤中天麻素和鸟苷含量测定的方法。方法:使用Platisil ODS色谱柱(4.6 mm×250 mm,5μm)柱;流动相A(水)-B(乙腈);梯度洗脱0～30 min(2%～5%B);流速0.65 mL·min-1;柱温25℃;检测波长260 nm。结果:天麻素和鸟苷分别在0.007～0.35 g·L-1(r=0.999 9)和0.000 32～0.016 g·L-1(r=0.999 9)内线性关系良好,平均回收率分别为99.7%和100.6%,RSD分别为1.9%和2.0%(n=9)。结论:所建立的方法准确快速,可用于半夏白术天麻汤复方的质量控制。
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| 关 键 词： | 复方半夏白术天麻汤 天麻素 鸟苷 高效液相色谱法 |
| 收稿时间： | 2010/5/13 0:00:00 |
Simultaneous Determination of Gastrodine and Guanosine in Banxia Baizhu Tianmatang |
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| WANG Xun,LU Jia-feng,LUO Shan-shan,WANG Xian-zhen,JIANG Jia-ye and KE Yan.Simultaneous Determination of Gastrodine and Guanosine in Banxia Baizhu Tianmatang[J].China Journal of Experimental Traditional Medical Formulae,2010,16(17):44-47. |
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| Authors: | WANG Xun LU Jia-feng LUO Shan-shan WANG Xian-zhen JIANG Jia-ye and KE Yan |
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| Institution: | Experiment Center For Teaching And Learning,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Experiment Center For Teaching And Learning,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Experiment Center For Teaching And Learning,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Experiment Center For Teaching And Learning,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Experiment Center For Teaching And Learning,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Experiment Center For Teaching And Learning,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China |
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| Abstract: | |
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| Keywords: | Banxia Baizhu Tianmatang gastrodine guanosine HPLC |
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