复方倍他米松搽剂中倍他米松含量的测定

目的：建立完善的高效液相色谱法,准确地测定复方倍他米松搽剂中倍他米松的含量。方法：采用Waters Symmetry C18（250 mm×4.6 mm,5μm）色谱柱,流动相为乙腈-水（25：75）,流速：1.0mL/min,柱温35℃,检测波长240nm。结果：倍他米松的线性范围为10～100μg/mL（r=0.9999）；平均加样回收率为101.8%,RSD为1.3%（n=9）。结论：该方法简便准确,为该制剂的质量控制提供可靠依据。

HPLC Determination of Betamethasone in Compound Betamethasone liniment

Objective： To establish an improved HPLC method for the accurate determination of betamethasone in compound betamethasone liniment. Methods： The chromatographic separation was performed on a Waters Symmetry C18 column with a mixture of acetonitrile and water（25：75 v/v）as mobile phase at the flow rate of 1.0mL/min. The column temperature was maintained at 35℃ and the detection wave length was 240 nm. Results： The calibration curves in the range of 10-100 μg/mL（r=0.9999） for betamethasone was linear. The average recovery was 101.8%, and RSD was 1.3%（n=9）. Conclusion： An accurate method can be used for the quality control of compound betamethasone liniment.