| 仙灵骨葆胶囊对去势大鼠骨组织H3K36me3、H3K9me3及JMJD2A表达的影响 |
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| 引用本文： | 王粤淇,霍少川,陈德龙,张濛,唐宏宇,王海彬. 仙灵骨葆胶囊对去势大鼠骨组织H3K36me3、H3K9me3及JMJD2A表达的影响[J]. 中国骨质疏松杂志, 2020, 0(1): 16-20 |
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| 作者姓名： | 王粤淇 霍少川 陈德龙 张濛 唐宏宇 王海彬 |
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| 作者单位： | 1.广州中医药大学第一临床医学院，广东 广州 5104052.广州中医药大学岭南医学研究中心中医骨伤科学实验室，广东 广州5104053.广州中医药大学第一附属医院关节骨科，广东 广州 510405 |
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| 基金项目： | 国家自然科学基金(81774339) |
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| 摘 要： |
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| 关 键 词： | 仙灵骨葆胶囊；福美加；JMJD2A；H3K36me3；H3K9me3；大鼠；动物实验；骨质疏松 |
Effect of Xianlinggubao capsule on H3K36me2, H3K9me2, and JMJD2A expression in bone tissues of ovariectomized rats |
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| WANG Yueqi,HUO Shaochuan,CHEN Delong,ZHANG Meng,TANG Hongyu,WANG Haibin. Effect of Xianlinggubao capsule on H3K36me2, H3K9me2, and JMJD2A expression in bone tissues of ovariectomized rats[J]. Chinese Journal of Osteoporosis, 2020, 0(1): 16-20 |
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| Authors: | WANG Yueqi HUO Shaochuan CHEN Delong ZHANG Meng TANG Hongyu WANG Haibin |
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| Affiliation: | 1.The First Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou 5104052.Laboratory of Orthopedics and Trauma of Traditional Chinese Medicine, Lingnan Medical Research Center, Guangzhou University of Traditional Chinese Medicine, Guangzhou 5104053.Department of Joint Orthopedics, the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China |
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| Abstract: | Objective To investigate the effect of Xianlinggubao capsule on the expression of H3K36me3 (histone H3 lysine 36 trimethylation), H3K9me3 (histone H3 lysine 9 trimethylation), and JMJD2A in bone tissues of ovariectomized rats. Methods Twenty-four female SD rats were randomly divided into four groups: blank control group, negative control group, experimental group, and positive control group, with 6 rats in each group. The rats in the blank control group retained bilateral ovaries and only some fat beside the ovaries were excised. Osteoporosis model was established in the other 3 groups of rats by resection of bilateral ovaries. One week after the operation, rats in the experimental group received 30.85 mg/100 g of Xianlinggubao capsule. Rats in the positive control group received 11.7 mg/100 g of Fumeijia. Rats in the blank control and the negative control group received the same volume of distilled water at the same time. After 3 months of continuous administration, HE staining was used to observe the microstructure of bone; RT-PCR was used to detect the mRNA expressions of histone H3 and JMJD2A. Western blotting (WB) was used to detect the protein expressions of H3K36me3, H3K9me3, and JMJD2A. Results The bone microstructure in the blank group, experimental group, and positive control group was superior to that in the negative control group. The expression of JMJD2A in bone tissue of blank group, experimental group, and positive control group was significantly higher than that in the negative control group (P<0.05). The expression of histone H3 mRNA in blank group, experimental group, and positive control group was lower than that in the negative control group (P<0.05). The protein expressions of H3K9me3 and H3K36me3 in the blank group, experimental group and positive control group were lower than that in the negative control group (P<0.05). Conclusion Xianlinggubao capsule relieves osteoporosis symptoms in ovariectomized rats. Its mechanism may be related to the increase of JMJD2A expression in bone tissues of ovariectomized rats and the inhibition of H3K36 me3 and H3K9 me3 expression. |
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| Keywords: | Xianlinggubao capsule Fumeijia JMJD2A H3K36me3 H3K9me3 rats animal experimentation osteoporosis |
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