| 芍药苷对人上皮性卵巢癌HO8910细胞增殖、凋亡及迁移的影响及其机制研究 |
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| 作者姓名： | 高天勤 李欣 董圣军 程春雷 史荣军 贾龙 王健 刘宝辉 |
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| 作者单位： | 1.滨州医学院附属医院，山东 滨州 256603,2.滨州市人民医院，山东 滨州 255610,1.滨州医学院附属医院，山东 滨州 256603,3.山东省食品药品检验研究院，山东 济南 250101,4.阳信县人民医院，山东 阳信 251800,1.滨州医学院附属医院，山东 滨州 256603,1.滨州医学院附属医院，山东 滨州 256603,1.滨州医学院附属医院，山东 滨州 256603 |
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| 摘 要： | 目的 观察芍药苷（Paeoniflorin，PF）对人上皮性卵巢癌HO8910细胞增殖、凋亡及迁移能力的影响，并探讨其可能的作用机制。方法 培养人上皮性卵巢癌HO8910细胞，随机分为对照组、芍药苷低剂量组、芍药苷中剂量组、芍药苷高剂量组。芍药苷低、中、高剂量组分别在加入0.5、1与2 mg·mL-1芍药苷的DMEM培养液中培养48 h。采用四甲基偶氮唑蓝法（MTT）检测HO8910细胞增殖抑制率；TUNEL染色法检测细胞凋亡率；细胞划痕实验检测HO8910细胞迁移率；免疫蛋白印迹技术（Western blot）分析核因子-κB（NF-κB) p56、Bcl-2、caspase-3蛋白的表达。结果 与对照组相比，芍药苷对HO8910细胞增殖、迁移率有明显的抑制作用并促进其凋亡（P<0.05），这种作用呈现剂量依赖性；芍药苷处理组细胞内caspase-3表达水平较对照组明显升高（P<0.05），Bcl-2、核因子-κB p56表达水平较对照组明显降低（P<0.05），且呈现剂量依赖性（P<0.05）。结论 芍药苷可明显抑制HO8910细胞的增殖，诱导其凋亡；其机制部分可能与阻断核因子-κB通路有关。
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| 关 键 词： | 芍药苷 细胞增殖 细胞凋亡 细胞迁移 核因子-κB信号通路 |
Effects of paeoniflorin on proliferation, apoptosis and migration of ovarian cancer cells HO8910 |
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| Authors: | GAO Tianqin LI Xin DONG Shengjun CHENG Chunlei SHI Rongjun JIA Long WANG Jian and LIU Baohui |
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| Institution: | 1.Affiliated Hospital of Binzhou Medical College, Binzhou 256603,China;2.Binzhou People''s Hospital, Binzhou 255610, China;3.Shandong Institute of Food and Drug Control, Jinan 250101,China;4.People''s Hospital of Yangxin County,Yangxin251800,China,1.Affiliated Hospital of Binzhou Medical College, Binzhou 256603,China;2.Binzhou People''s Hospital, Binzhou 255610, China;3.Shandong Institute of Food and Drug Control, Jinan 250101,China;4.People''s Hospital of Yangxin County,Yangxin251800,China,1.Affiliated Hospital of Binzhou Medical College, Binzhou 256603,China;2.Binzhou People''s Hospital, Binzhou 255610, China;3.Shandong Institute of Food and Drug Control, Jinan 250101,China;4.People''s Hospital of Yangxin County,Yangxin251800,China,1.Affiliated Hospital of Binzhou Medical College, Binzhou 256603,China;2.Binzhou People''s Hospital, Binzhou 255610, China;3.Shandong Institute of Food and Drug Control, Jinan 250101,China;4.People''s Hospital of Yangxin County,Yangxin251800,China,1.Affiliated Hospital of Binzhou Medical College, Binzhou 256603,China;2.Binzhou People''s Hospital, Binzhou 255610, China;3.Shandong Institute of Food and Drug Control, Jinan 250101,China;4.People''s Hospital of Yangxin County,Yangxin251800,China,1.Affiliated Hospital of Binzhou Medical College, Binzhou 256603,China;2.Binzhou People''s Hospital, Binzhou 255610, China;3.Shandong Institute of Food and Drug Control, Jinan 250101,China;4.People''s Hospital of Yangxin County,Yangxin251800,China,1.Affiliated Hospital of Binzhou Medical College, Binzhou 256603,China;2.Binzhou People''s Hospital, Binzhou 255610, China;3.Shandong Institute of Food and Drug Control, Jinan 250101,China;4.People''s Hospital of Yangxin County,Yangxin251800,China and 1.Affiliated Hospital of Binzhou Medical College, Binzhou 256603,China;2.Binzhou People''s Hospital, Binzhou 255610, China;3.Shandong Institute of Food and Drug Control, Jinan 250101,China;4.People''s Hospital of Yangxin County,Yangxin251800,China |
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| Abstract: | |
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| Keywords: | Paeoniflorin Cell proliferation Cell migration Cell apoptosis NF-nuclear factor-kappaB signal pathway |
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