| 星点设计-效应面法优化壳聚糖微球对凝血酶的固定化作用研究 |
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| 作者姓名： | 苗艳丽 李卫东 钟丽兰 廖铭能 陈绍红 李翠娟 林子诚 |
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| 作者单位： | 1.广东海洋大学化学与环境学院，广东 湛江 524088,1.广东海洋大学化学与环境学院，广东 湛江 524088,1.广东海洋大学化学与环境学院，广东 湛江 524088,1.广东海洋大学化学与环境学院，广东 湛江 524088,2.广东海洋大学食品科技学院现代生物化学实验中心，广东 湛江 524088,1.广东海洋大学化学与环境学院，广东 湛江 524088,1.广东海洋大学化学与环境学院，广东 湛江 524088 |
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| 基金项目： | 广东省教育厅重点平台及科研项目（No.2015KTSCX057）；广东省自然科学基金（No.2016A030308009）；广东海洋大学“海之帆—起航计划”大学生科技创新培育项目（No.hzfqhjhzrkx2015b07）；广东海洋大学大学生创新创业训练项目（No.CXXL2017140） |
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| 摘 要： | 目的 考察壳聚糖微球对凝血酶的固定化作用。方法 本研究以壳聚糖微球为载体，以戊二醛为交联剂将凝血酶固定于空白微球上，以固定化凝血酶的活性回收率为指标，采用星点设计-效应面法优化固定凝血酶的条件。结果 试验结果表明，壳聚糖微球固定凝血酶的最优条件是：凝血酶浓度69.32 U·mL-1、戊二醛浓度0.18%、固定化时间1.88 h、固定化pH 7.08，凝血酶的活性回收率为81.55 %。结论 壳聚糖微球对凝血酶的固定化效果良好。
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| 关 键 词： | 凝血酶 壳聚糖微球 固定化 活性回收率 星点设计-效应面法 |
Optimization of immobilized effect of chitosan microspheres on thrombin by the central composite design-response surface methodology |
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| Authors: | MIAO Yanli LI Weidong ZHONG Lilan LIAO Mingneng CHEN Shaohong LI Cuijuan and LIN Zicheng |
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| Institution: | 1.College of Chemistry and Environment of Guangdong Ocean University, Zhanjiang 524088,China;2.College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University,Zhanjiang 524088,China,1.College of Chemistry and Environment of Guangdong Ocean University, Zhanjiang 524088,China;2.College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University,Zhanjiang 524088,China,1.College of Chemistry and Environment of Guangdong Ocean University, Zhanjiang 524088,China;2.College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University,Zhanjiang 524088,China,1.College of Chemistry and Environment of Guangdong Ocean University, Zhanjiang 524088,China;2.College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University,Zhanjiang 524088,China,1.College of Chemistry and Environment of Guangdong Ocean University, Zhanjiang 524088,China;2.College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University,Zhanjiang 524088,China,1.College of Chemistry and Environment of Guangdong Ocean University, Zhanjiang 524088,China;2.College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University,Zhanjiang 524088,China and 1.College of Chemistry and Environment of Guangdong Ocean University, Zhanjiang 524088,China;2.College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University,Zhanjiang 524088,China |
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| Abstract: | |
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| Keywords: | Thrombin Chitosan microspheres Immobilization Activity yield Central composite design-response surface methodology |
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