|
|||||
|
|
| 食管鳞癌患者血清RUNX3基因甲基化检测及临床意义 | |
| 引用本文： | 郑芸,张有为,陈龙邦. 食管鳞癌患者血清RUNX3基因甲基化检测及临床意义[J]. 癌症进展, 2010, 8(3): 290-294. DOI: 10.3969/j.issn.1672-1535.2010.03.021 |
| 作者姓名： | 郑芸 张有为 陈龙邦 |
| 作者单位： | 第二军医大学南京临床医学院,南京军区南京总医院肿瘤内科,南京210002;第二军医大学南京临床医学院,南京军区南京总医院肿瘤内科,南京210002;第二军医大学南京临床医学院,南京军区南京总医院肿瘤内科,南京210002 |
| 摘 要： | 目的检测食管鳞癌患者血清中RUNX3基因启动子区域甲基化状态，探讨用于食管鳞癌早期诊断和预后评估的临床意义。方法留取70例食管鳞癌，20例食管良性病变及10例健康志愿者血清标本，甲基化特异性聚合酶链反应（MSP）分析RUNX3基因启动子区域甲基化情况，并分析其与临床病理参数之间的相关性。结果70例食管鳞癌患者血清RUNX3基因启动子区域异常甲基化36例，检出率为51．4％，20例食管良性病变患者中有2例为不完全甲基化（10％），而10例健康志愿者中检出率为0，差异有统计学意义（P〈0．001）；RUNX3基因启动子甲基化与患者临床分期和淋巴结转移相关。结论RUNX3基因启动子甲基化在食管鳞癌患者血清中有着较高的检出率，可望成为食管鳞癌早期诊断和预后评估的分子标志物。 |
| 关 键 词： | 食管肿瘤 RUNX3 甲基化 分子标志物 循环DNA |
Analysis of RUNX3 promoter hypermethylation in serum DNA of esophageal squamous cell carcinoma patients |
|
| Zheng Yun,Zhang Youwei,Chen Longbang. Analysis of RUNX3 promoter hypermethylation in serum DNA of esophageal squamous cell carcinoma patients[J]. Oncology Progress, 2010, 8(3): 290-294. DOI: 10.3969/j.issn.1672-1535.2010.03.021 | |
| Authors: | Zheng Yun Zhang Youwei Chen Longbang |
| Affiliation: | Zheng Yun Zhang Youwei Chen Longbang~# Department of Medical Oncology,Nanjing Clinical School of the Second Military Medical University,Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 210002,China. |
| Abstract: | Objective To detect the promoter methylation status of RUNX3 gene in serum DNA of esophageal squamous cell carcinoma(ESCC) patients and evaluate its correlation with clinicopathological features.Methods Serum DNA was extracted from peripheral blood of 70 ESCC patients and 30 controls of benign esophageal diseases or healthy donors.Promoter methylation status of RUNX3 gene was determined by methylation-specific polymerase chain reaction(MSP),and the correlation between methylation profiles and clinicopatholo... |
| Keywords: | RUNX3 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |