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| MSRE-PCR法检测卵巢癌组织中OPCML基因甲基化 | |
| 引用本文： | 周峰,曹兴建,马敏,刘曼华,陶国华. MSRE-PCR法检测卵巢癌组织中OPCML基因甲基化[J]. 临床检验杂志, 2011, 29(6): 507-509 |
| 作者姓名： | 周峰 曹兴建 马敏 刘曼华 陶国华 |
| 作者单位： | 南通大学第二附属医院检验科，江苏南通 226001;南通大学第二附属医院检验科，江苏南通 226001;南通大学第二附属医院检验科，江苏南通 226001;南通大学第二附属医院妇产科，江苏南通 226001;南通大学第二附属医院检验科，江苏南通 226001 |
| 基金项目： | 南通市社会发展科技项目 (S2009017)。 |
| 摘 要： | 目的 建立甲基化敏感性限制性内切酶-PCR(MSRE-PCR)法检All OPCML基因,探讨其在卵巢癌中的意义.方法 用亚硫酸氛钠修饰测序法检测5例卵巢癌组织及正常卵巢组织中OPCML基因甲基化,分别用MSRE-PCR和巢式甲基化特异性PCR(MSP)检测组织DNA中OPCML基因甲基化发生率.结果 DNA序列中存在... |
| 关 键 词： | 甲基化敏感性限制性内切酶 聚合酶链反应 结合鸦片样物质的细胞黏附分子基因 卵巢癌 |
| 收稿时间： | 2011-02-01 |
| 修稿时间： | 2011-05-24 |
Detection of OPCML methylation in tissues of ovarian cancer by MSRE-PCR |
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| ZHOU Fenga,CAO Xing-jiana,MA Mina,LIU Man-huab,TAO Guo-huaa. Detection of OPCML methylation in tissues of ovarian cancer by MSRE-PCR[J]. Chinese Journal of Clinical Laboratory Science, 2011, 29(6): 507-509 | |
| Authors: | ZHOU Fenga CAO Xing-jiana MA Mina LIU Man-huab TAO Guo-huaa |
| Affiliation: | ZHOU Fenga,CAO Xing-jiana,MA Mina,LIU Man-huab,TAO Guo-huaa(a.Department of Clinical Laboratory,b.Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu,China) |
| Abstract: | Objective To establish the method of methylation-sensitive restriction enzyme-PCR(MSRE-PCR) and investigate the significance of detection of opioid-binding cell adhesion molecule(OPCML) methylation in ovarian cancer.Methods MSRE-PCR for OPCML was established on the base of the known OPCML methylation status.The methylation rates of OPCML in the cancer tissues and normal tissues of 5 cases with ovarian cancer were detected by bisulfite sequencing method and the established MSRE-PCR.Results Six Bsh1236I diges... |
| Keywords: | methylation-sensitive restriction enzyme PCR opioid-binding cell adhesion molecule ovarian cancer |
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