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| 固相萃取-高效液相色谱法测定甘草中甘草苷和甘草酸含量 | |
| 引用本文： | 张文娟,房敏峰,赵新锋,李云峰,郑晓晖,王亚洲. 固相萃取-高效液相色谱法测定甘草中甘草苷和甘草酸含量[J]. 中国药业, 2008, 17(17): 5-6 |
| 作者姓名： | 张文娟 房敏峰 赵新锋 李云峰 郑晓晖 王亚洲 |
| 作者单位： | 1. 西北大学生命科学学院,陕西,西安,710069 2. 西北大学中草药现代化研究及工程中心,陕西,西安,710069 3. 西北大学生命科学学院,陕西,西安,710069;西北大学中草药现代化研究及工程中心,陕西,西安,710069 |
| 基金项目： | 陕西省教育厅资助项目，陕西省重大科技专项资助项目 |
| 摘 要： | 目的探讨用固相萃取-高效液相色谱（SPE—HPLC）法测定甘草中甘草苷和甘草酸含量。方法采用正交试验法考察真空度、上样量、洗脱剂用量和洗脱流速对固相萃取工艺的影响，用高效液相色谱梯度洗脱法测定各萃取液中甘草苷和甘草酸含量。结果甘草最佳固相萃取备件为真空度0．01MPa，上样量1．5mL，洗脱剂用量25mL，洗脱速率3mL／min；甘草苷和甘草酸的方法回收率分别为100．85％和99．67％。结论SPE—HPLC法操作简单，准确可靠，可用于甘草及含甘草的复方制剂中甘草苷和甘草酸含量测定． |
| 关 键 词： | 甘草 甘草苷 甘草酸 固相萃取-高效液相色谱法 |
Determination of Content of Liquiritin and Glycyrrhizic Acid in Radix Glycyrrhizae by SPE-HPLC |
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| Zhang Wenjuan,Fang Minfeng,Zhao Xinfeng,Li Yunfeng,Zheng Xiaohui,Wang Yazhou. Determination of Content of Liquiritin and Glycyrrhizic Acid in Radix Glycyrrhizae by SPE-HPLC[J]. China Pharmaceuticals, 2008, 17(17): 5-6 | |
| Authors: | Zhang Wenjuan Fang Minfeng Zhao Xinfeng Li Yunfeng Zheng Xiaohui Wang Yazhou |
| Affiliation: | Zhang Wenjuan, Fang Minfeng, Zhao Xinjeag, Li Yunfeng, Zheng Xiaohui, Wang Yazhou( 1. College of Life Science, Northwest University, Xi＇an, Shaanxi, China 710069; 2. Research and Engineering Center of Modernization of Chinese Herbal Medicine, Xi＇ an, Shaanxi, China 710069) |
| Abstract: | Objective To study the detection method for liquiritin and glycyrrhizic acid in Radix Glycyrrhizae by solid phase extraction(SPE) and HPLC.Methods The orthogonal design was used to investigate the effect of four factors,including vacuum tightness,sample size,amount of eluent and eluting rate,on the solid phase extract technology.Liquiritin and the glycyrrhizic acid in various extract solutions were detected by gradient elution and HPLC.Results The optimized conditions of solid phase extract were as follows:the vacuum tightness was 0.01 MPa,the sample size was 1.5 mL,the amount of eluent was 25 mL and eluting rate was 3.0 mL/min.The recovery rate of liquiritin and glycyrrhizic acid was 100.85%and 99.67%respectively.Conclusion The established method is simple,reliable and suitable for the determination of liquiritin and glycyrrhizic acid in Radix Glycyrrhizae and compound preparations containing glycyrrhiza. |
| Keywords: | Radix Glycyrrhizae liquiritin glycyrrhizic acid SPE-HPLC |
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