Analytical characterization of an assay designed to detect and identify diverse agents of disseminated viral infection |
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| Affiliation: | 1. Shanghai Public Health Clinical Center & Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology of Ministry of Education/Health, Shanghai Medical College, Fudan University, Shanghai, China;2. State Key Laboratory for Infectious Disease Prevention and Control, China CDC, Beijing, China;1. Department of First Critical Care, Evangelismos Hospital, School of Medicine, National University of Athens, Athens, Greece;2. Department of Hematology, Evangelismos Hospital, Athens, Greece;3. Department of Pathology, Evangelismos Hospital, Athens, Greece;4. Department of Pathophysiology, Laiko Hospital, School of Medicine, National University of Athens, Athens, Greece;1. Y.R. Gaitonde Centre for AIDS Research and Education, Voluntary Health Services Hospital Campus, Taramani, Chennai 600113, India;2. Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;1. Department of Transplant Medicine, University Hospital Münster, Münster, Germany;2. Department of General and Visceral Surgery, University Hospital Münster, Münster, Germany;1. Department of Clinical Chemistry, Microbiology and Immunology, University Hospital Ghent, De Pintelaan 185, 9000 Ghent, Belgium;2. Department of Microbiology & Immunology, KU Leuven and Clinical Department Laboratory Medicine University Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium;3. School of Life Sciences, Hasselt University, Agoralaan Building D, 3590 Diepenbeek, Belgium;1. North Shore-LIJ Laboratories, Lake Success, NY, United States;2. Ann and Robert H. Lurie Children''s Hospital of Chicago, Northwestern University Feinberg School of Medicine, Chicago, IL, United States;3. Molecular Diagnostics Laboratory, Le Bonheur Children''s Hospital, Memphis, TN, United States;4. Departments of Pediatrics and Microbiology, Immunology, and Molecular Biology, University of Tennessee Health Science Center, Memphis, TN, United States;5. Feinstein Institute for Medical Research, Hofstra North Shore-LIJ School of Medicine, Hempstead, NY, United States |
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| Abstract: | ![]()
BackgroundDiverse viruses often reactivate in or infect cancer patients, patients with immunocompromising infections or genetic conditions, and transplant recipients undergoing immunosuppressive therapy. These infections can disseminate, leading to death, transplant rejection, and other severe outcomes.ObjectivesTo develop and characterize an assay capable of inclusive and accurate identification of diverse potentially disseminating viruses directly from plasma specimens.Study designWe developed a PCR/electrospray ionization mass spectrometry (PCR/ESI-MS) assay designed to simultaneously detect and identify adenovirus, enterovirus, polyomaviruses JC and BK, parvovirus B19, HSV-1, HSV-2, VZV, EBV, CMV, and herpesviruses 6–8 in plasma specimens. The assay performance was characterized analytically, and the results from clinical plasma samples were compared to the results obtained from single-analyte real time PCR tests currently used in clinical practice.ResultsThe assay demonstrated sensitivity and specificity to diverse strains of the targeted viral families and robustness to interfering substances and potentially cross reacting organisms. The assay yielded 94% sensitivity when testing clinical plasma samples previously identified as positive using standard-of-care real-time PCR tests for a single target virus (available samples included positive samples for 11 viruses targeted by the assay).ConclusionsThe assay functioned as designed, providing simultaneous broad-spectrum detection and identification of diverse agents of disseminated viral infection. Among 156 clinical samples tested, 37 detections were made in addition to the detections matching the initial clinical positive results. |
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| Keywords: | Viremia Molecular diagnostics Disseminated infection Clinical virology Immunocompromised Antiviral |
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