| 口腔念珠菌带菌与感染状态分离菌株的RAPD分析 |
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| 引用本文： | 周菊芬,徐岩英,李若瑜. 口腔念珠菌带菌与感染状态分离菌株的RAPD分析[J]. 实用口腔医学杂志, 2004, 20(2): 223-225 |
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| 作者姓名： | 周菊芬 徐岩英 李若瑜 |
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| 作者单位： | 1. 北京大学口腔医学院中医黏膜科,100081
2. 北京大学第一医院真菌和真菌病研究中心 |
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| 基金项目： | 本研究部分得到联合国教科文组织(UNESCO)东南亚地区微生物和生物技术网络资助 |
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| 摘 要： | 目的 :研究念珠菌种之间及不同来源白念珠菌菌株之间的基因型相关性。方法 :对口腔白念珠菌病原菌、共生菌及非白念病原菌分别进行表型和RAPD分析。结果 :白念珠菌与非白念珠菌的RAPD带型存在差异 ,相似系数在 5 1.7%。共生菌与病原菌RAPD带型未见差异。白念珠菌种内相似系数大于 70 % ,菌株间存在DNA多态性。结论 :共生白念珠菌与病原菌未见RAPD带型差别 ,提示对口腔念珠菌病的防治应重视宿主因素在发病中的作用。
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| 关 键 词： | 白念珠菌 随机扩增DNA多态性(RAPD) 口腔 |
| 文章编号： | 1001-3733(2004)-02-0223-03 |
| 修稿时间： | 2003-02-08 |
Random amplified polymorphic DNA analysis of candida isolates from oral carriage |
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| Zhou Jufen,Xu Yanying,Li Ruoyu. Random amplified polymorphic DNA analysis of candida isolates from oral carriage[J]. Journal of Practical Stomatology, 2004, 20(2): 223-225 |
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| Authors: | Zhou Jufen Xu Yanying Li Ruoyu |
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| Affiliation: | Zhou Jufen,Xu Yanying,Li Ruoyu.Department of Oral Medicine,Peking University School of Stomatology,Beijing 100081,China |
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| Abstract: | Objective: To compare the biotype and genetic similarity between groups of commensal and pathogenic strains of candida species. Methods:Random amplified polymorphic DNA (RAPD) was used to analyse the type of Candida albicans. Results: 12 pathogenic isolates of Candida albicans, 3 commensal isolates of Candida abicans and 3 isolates of pathogenic non-albicans were obtained. The similarity coefficient of albicans with non-albicans was 51.7% by RAPD. Intra-candida similarity coefficient was more than 70%. Both the commensal and pathogenic isolates showed the genetically similar to C. albicans. Intro-isolate DNA polymorphism was observed by RAPD. Conclusion: Both the commensal and pathogenic groups contain a major cluster of genetically similar C. albicans isolates. |
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| Keywords: | Candida albicans Random ampified polymorphic DNA Mouth |
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