| 沙眼衣原体感染与分泌性中耳炎的关系 |
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| 引用本文： | 沈宝茗,江青山,廖剑绚,谭凤君,唐洪波.沙眼衣原体感染与分泌性中耳炎的关系[J].临床耳鼻咽喉头颈外科杂志,2007,21(13):590-592. |
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| 作者姓名： | 沈宝茗 江青山 廖剑绚 谭凤君 唐洪波 |
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| 作者单位： | 南华大学第一附属医院耳鼻咽喉科,湖南衡阳,421001 |
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| 基金项目： | 湖南省教育厅科研基金项目（No：06C697） |
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| 摘 要： | 目的：探讨沙眼衣原体（Ct）感染与分泌性中耳炎（SOM）发病的关系。方法：PCR检测157例（183耳）SOM患者的中耳积液（MEE），用荧光定量PCR（FQ-PCR）对阳性标本进行定量检测，用McCoy细胞对其中含高拷贝数基因的标本进行Ct分离培养。结果：PCR检测的183份MEE标本中，29份为阳性，FQ-PCR检测这些阳性标本基因拷贝数在2．0×10^6／L～6．0×10^10／L，11耳高拷贝数基因的标本分离到1株Ct。结论：Ct感染与SOM发病有关，可能是导致SOM的致病微生物之一。
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| 关 键 词： | 沙眼衣原体 中耳炎 伴渗出液 |
| 文章编号： | 1001-1781（2007）13-0590-03 |
| 修稿时间： | 2006-12-19 |
The relationship between chlamydia trachomatis infection and secretory otitis media |
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| SHEN Baoming,JIANG Qingshan,LIAO Jianxuan,TAN Fengjun,TANG Hongbo.The relationship between chlamydia trachomatis infection and secretory otitis media[J].Journal of Clinical Otorhinolaryngology,2007,21(13):590-592. |
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| Authors: | SHEN Baoming JIANG Qingshan LIAO Jianxuan TAN Fengjun TANG Hongbo |
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| Institution: | Department of Otorhinolaryngology, the First Affiliated Hospital of Nanhua University, Hengyang, 421001 ,China |
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| Abstract: | Objective:To assess the relationship between the infection of chlamydia trachomatis(Ct) and secretory otitis media(SOM).Method:Deoxyribonucleic acid(DNA) of chlamydia trachomatis in 183 middle ear effusions(MEES)of SOM patients were detected by polymerase chain reaction(PCR) technique.Subsequently,the positive samples were detected by fluorescence quantitative PCR(FQ-PCR) technique.Lastly,chlamydia trachomatis in MEEs was separated by McCoy cell culture.Result:The results of PCR and FQ-PCR detection were the same that twenty-nine(15.85%) cases of the samples were Ct positive.One of these 11 MEEs was found Ct positive with culture.Conclusion:Infection of chlamydia trachomatis play a major role in the pathogenesis of otitis media with effusion. |
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| Keywords: | Chalmdiae trachomatis Otitis media with effusion |
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