鼻咽癌的基因不稳定性

目的:探讨鼻咽癌基因不稳定性对预后的意义.方法:运用单一重复序列间多聚酶链反应(inter-SSR PCR)对38位鼻咽癌患者基因不稳定性进行评估;运用测序和巢式PCR对不同肿瘤的突变片段进行检测.结果:38位患者中有31位(81.6%)显示了基因组失稳,其失稳指数范围为0%～16.2%.6个肿瘤的6q27染色体上证实有获得性基因损害.低于5年生存期患者其基因失稳程度显著高于超过5年生存期的患者(P＜0.05).结论:基因组失稳可作为鼻咽癌发生的早期标志,并且基因组失稳程度加重提示肿瘤预后不良.

Genomic instability in nasopharyngeal carcinoma

Objective To evaluate the effect of genomic instability on prognostics in nasopharyngeal carcinoma. Methods Genomic instability was assessed by inter-simple sequence repeats polymerase chain reaction (inter-SSR PCR) in 38 patients with nasopharyngeal carcinoma. Characterization and verification of band alterations shared in different tumors were carried out by sequencing and nest PCR. Results Thirty-one (81.6%) of the 38 patients showed genomic alteractions, and genomic instability index ranged 0 to 16.2 percent. A gain-based genomic damage shared in 6 tumors was identified on chromosome 6q27. Genomic alteration was significantly more in patients less than 5-year survival than those with more than 5-year survival (P<0.05). Conclusion Genomic instability can be an early event marker in carcinogenesis of nasopharyngeal carcinoma. Aggravation of genomic alterations is a poor prognosis for cancer recovery.