两种自制支架材料对体外培养关节软骨细胞生长与代谢的影响

目的 评定两种自制支架材料DL－聚乳酸支架,聚磷酸钙纤维对体外培养关节软骨细胞生长与代谢的影响。方法 采用材料样品与体外单层培养关节软骨细胞直接接触法,并对其进行倒置显微镜观察,培养细胞计数与DAN含量及其细胞外基质^35S掺入量和羟脯氨酸含量测定。结果 两种支架材料可与培养关节软骨细胞完全相容,无关节软骨细胞毒性;对培养关节软骨细胞的DNA,蛋白多糖及胶原合成无异常影响。结论 两种支架材料具有良好的关节软骨细胞相容性,对其功能物质的合成无异常影响,经其结构与性能优化,即可满足关节软骨组织工程制备的各项要求。

Effects of 2 scaffold materials on growth and metabolism of articular cartilage cells in monolayer culture

Objective To evaluate the effect of 2 scaffold materials, poly-DL-lactic acid and calcium polyphosphate fibers, for the articular cartilage tissue engineering on growth and metabolism of articular cartilage cells in monolayer culture. Methods Converted microscopy, cell counting, total DNA assay and measurement of 35 S incorporation and hydrooxyprotine in cultured articular cartilage cells which were directly contacted with the scaffold materials were studied. Results Two scaffold materials showed entire biocompatibility with cultured chondrocytes and no chondrocyto-toxicity was observed. They had no influence on the synthesis of DNA, proteoglycan and collagen of chondrocytes in monolayer culture. Conclusion These 2 scaffold materials have not only excellent chondrocyte biocompatibility but also no effect on the synthesis of functional substances of chondrocytes in monolayer culture. After further optimization in their structure and properties, they can be regarded as scaffold materials to satisfy the articular cartilage tissue engineering.