10%水合氯醛与2%戊巴比妥钠在建立术后疲劳综合征大鼠模型中的麻醉效果比较

目的 比较10%水合氯醛与2%戊巴比妥钠在建立术后疲劳综合征(POFS)大鼠模型中的麻醉效果.方法 采用70%中段小肠切除后端端吻合法建立术后疲劳综合征大鼠模型.将SPF级健康雄性大鼠24只,随机分为10%水合氯醛组(A组)、2%戊巴比妥钠组(B组)麻醉,观察比较麻醉指标(麻醉死亡率、麻醉状态、麻醉时间、首剂麻醉成功率),术后疲劳综合征评测指标(术前、术后6 h、2、4、6、8 d大鼠的肛温,术后第2~7天进行水迷宫实验结果,术后1、3、5、7 d测试最大抓力结果).结果 两组大鼠的死亡率未见明显差异,与B组比较,A组麻醉诱导时间和深度麻醉时间更短(P<0.05),术后肛温恢复更快(P<0.05),最大抓力在术后第1、3、5、7天明显更大(P<0.05),在水迷宫实验中总平均逃避潜伏期明显更短(P<0.05),穿越平台次数明显更多(P<0.05).结论 在POFS大鼠模型制备中,与2%戊巴比妥相比较,采用10%水合氯醛麻醉,麻醉诱导时间更短,深度麻醉时间适中,首剂麻醉成功率高.麻醉可能对POFS的程度有一定影响,选用10%水合氯醛作为麻醉剂,大鼠模型的POFS程度更轻,质量控制较好.

Anesthesia effects of chloral hydrate and pentobarbital sodium on postoperative fatigue syndrome in rats

Objective To compare the anesthesia effects of chloral hydrate (10%) and pentobarbital sodium (2%) on postoperative fatigue syndrome (POFS) model in rats.Methods Rat model of POFS were established by with enterectomy with anastomosis method.Twenty four adult male healthy rats were randomly divided into 2 groups, chloral hydrate group and pentobarbital sodium group.The durations of anesthesia induction and anesthesia duration, rat mortality, and success rate of the first dose of anesthesia were recorded.Anal temperature was measured at baseline, and 6 hours, 2 days, 4 days, 6 days and 8 days after operation.Morris water-maze test was carried out 2-7 dyas after operation.Meanwhile, grasping test was applied 1 day, 3 days, 5 days and 7 days after operation.Results There was no significant difference in mortality between the two groups.The induction time and deep anesthesia time in chloral hydrate (P<0.05), in which the rectal temperature was regained significantly faster.The maximum grips were significantly greater on Day 1, 3, 5 and 7 in chloral hydrate group (P<0.05), with significantly shorter escape latency and more platform crossing times (P<0.05).Conclusion Chloral hydrate can provide shorter induction time, satisfied duration, and higher the success rate of the first dose of anesthesia, suitable in constructing the POFS model.