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| 单克隆抗体竞争性ELISA在日本血吸虫病诊断中的应用 | |
| 引用本文： | 娄文娴 杨士静. 单克隆抗体竞争性ELISA在日本血吸虫病诊断中的应用[J]. 现代免疫学, 1990, 10(5): 295-298 |
| 作者姓名： | 娄文娴 杨士静 |
| 作者单位： | 上海第二医科大学寄生虫学教研室，上海第二医科大学寄生虫学教研室，上海第二医科大学寄生虫学教研室，上海市免疫学研究所寄生虫感染免疫研究室，上海市免疫学研究所寄生虫感染免疫研究室 200025，200025，200025 |
| 基金项目： | “七五”国家科技攻关专题基金 |
| 摘 要： | 本文应用单克隆抗体ⅢD10竞争性ELISA,对369例粪检阳性日本血吸虫病患者(慢性316例,急性54例),检测血清内抗CCA 抗体水平,并与环卵沉淀试验和间接血凝试验对比。结果表明,C-ELISA 的敏感性为 97.75～100％,高于 CCPT(90.27％,p<0.05)。而正常人无假阳性,与肝吸虫、肺吸虫和包虫感染的突叉反应依次为1.39％、1/18和0。特异性明显高于COPT和IHA。本试验快速、简便、重复性好。 |
| 关 键 词： | 单克隆抗体 ESISA 血吸虫病 诊断 |
APPLICATION OF MONOCLONAL ANTIBODY COMPETITIVE ELISA IN DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA |
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| Abstract: | 369 confirmed (stool positive) schistosomiasis cases (acute, 54; chronic, 315) and 526 normal controls were detected for anti-CCA antibody levels in sera by monoclonal antibody IIID 10 based C-ELISA, the results were compare with those of COPT and IHA. Positive reactions were obtained in 97.75-100% of documented patients' sera with none in the controls. The cross reaction rates with sera from patients of clonorchi-asis, paragonimiasis and hydatid diseases were 1.39%, 1/18 and O respectively. The specificity in C-ELISA was shown to be significantly better than that in COPT or IHA, while the sensitivity was superior to COPT. C-ELISA was found to be simple, rapid and reproducible. |
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| 本文献已被 CNKI 维普 等数据库收录！ | |