间接法原位PCR检测喉鳞癌组织HPV感染

建立稳定的原位ＰＣＲ方法,并探讨ＨＰＶ感染与喉鳞癌发生的关系。方法采用免疫组化、原位杂变、ＰＣＲ和间接原位ＰＣＲ技术,检测了５０例喉鳞癌中的ＨＰＶ感染情况。结果免疫组化衣壳抗原阳性者６例（１２％）,原位杂交阳性者１３例（２６％）,ＰＣＲ阳性者１０例（２０％）,原位ＰＣＲ阳性者１７例（３４％）,综合上述方法的检出率为４２％（２１例）。结论ＨＰＶ感染与喉癌有着明显的关系,间接原位ＰＣＲ在检测ＨＰＶ感染

Detectionof HPV infection in larynx squamous cell carcinoma tissues by indirect in situ polymerasechain reaction

Aim To make up a stable method of in situ PCR and to probe the relationship between HPV infection and larynx squamous cell carcinomas. Metheds HPV infection in 50 specimens of larynx squamous cell carcinoma was examed by immunohistochemical staining. in situ hybridization,PCR and indirect in situ PCR. Results The positive detection rate of HPV DNA was 12%by immunohistochemistry, 26%by in situ hybridization,20%by PCR, 34%by in situ PCR, and totally 42%in twenty one out of fifty cases. Conclusion HPV infection is related to the occurrence of larynx squamous cell carcinomas. Indirect in situ PCR has better sensitivity,reliability, and specificity than other methods. It is reliable and has the role of diagnosis of localization and nature of the lesion.