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| 大鼠鼻黏膜外胚层间充质干细胞向成骨细胞和神经元的诱导分化 | |
| 引用本文： | 贺清华,周月鹏,陈谦,刘锦波,吴滢,庄琴,孙湘兰,龚爱华,姜平,张志坚. 大鼠鼻黏膜外胚层间充质干细胞向成骨细胞和神经元的诱导分化[J]. 江苏大学学报(医学版), 2011, 21(3): 190-193,180 |
| 作者姓名： | 贺清华 周月鹏 陈谦 刘锦波 吴滢 庄琴 孙湘兰 龚爱华 姜平 张志坚 |
| 作者单位： | 江苏大学基础医学与医学技术学院,江苏,镇江,212013 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的:建立鼻黏膜外胚层间充质干细胞(ectomesenchymal stem cells,ECTO-MSCs)的体外培养方法,探讨该细胞的干细胞特性(stemness)和分化潜能.方法:在观察ECTO-MSCs在大鼠鼻腔呼吸黏膜内分布特征的基础上,体外培养扩增ECTO-MSCs,并用干细胞标志蛋白巢蛋白、CD133、C... |
| 关 键 词： | 鼻黏膜 外胚层间充质干细胞 诱导分化 成骨细胞 神经细胞 |
Induced differentiation of ectomesenchymal stem cells derived from rat nasal mucosa to osteoblasts and neurons |
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| HE Qing-hua,ZHOU Yue-peng,CHEN Qian,LIU Jin-bo,WU Ying,ZHUANG Qin,SUN Xiang-lan,GONG Ai-hua,JIANG Ping,ZHANG Zhi-jian. Induced differentiation of ectomesenchymal stem cells derived from rat nasal mucosa to osteoblasts and neurons[J]. Journal of Jiangsu University Medicine Edition, 2011, 21(3): 190-193,180 | |
| Authors: | HE Qing-hua ZHOU Yue-peng CHEN Qian LIU Jin-bo WU Ying ZHUANG Qin SUN Xiang-lan GONG Ai-hua JIANG Ping ZHANG Zhi-jian |
| Affiliation: | HE Qing-hua,ZHOU Yue-peng,CHEN Qian,LIU Jin-bo,WU Ying,ZHUANG Qin,SUN Xiang-lan,GONG Ai-hua,JIANG Ping,ZHANG Zhi-jian(School of Medical Science and Laboratory Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China) |
| Abstract: | Objective: To establish an in vitro method to culture ectomesenchymal stem cells(ECTO-MSCs) derived from nasal mucosa,and explore their stemness and differentiation potential.Methods: Based on the observation of distribution of ECTO-MSCs in rat nasal mucosa,we cultured and proliferated ECTO-MSCs in vitro and identified the expression of stem cell markers on them including nestin,CD133,CD44 and Vimentin with immunofluorescence.Besides,osteogenesis induced media,with ingredient of dexamethasone,ascorbic acid ... |
| Keywords: | nasal mucosa ectomesenchymal stem cells induced differentiation osteoblasts neuron |
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