茶多酚对60Co γ辐射诱发CHL细胞染色体畸变和微核形成作用的影响

目的 研究茶多酚（tea polyphenols，TP）对60Coγ辐射诱发中国仓鼠肺成纤维细胞（Chinese hamster fibroblast cell line，CHL）染色体畸变和小鼠骨髓微核形成作用的影响。方法 将CHL细胞（±S9）分为空白对照组、阳性对照组、辐射模型组，TP保护组（分别加TP12.5、25、50 μg/ml）6组，除空白和阳性对照组外均给予2 Gy ⁶⁰Co γ照射后继续培养24 h，常规制备染色体，观察畸变类型并计算畸变率。50只KM小鼠分为正常对照组、辐射模型组，TP保护组（高、中、低剂量分别于照射后给TP725、145、29 mg/kg灌胃），每组10只，给药后14天给予6 Gy ⁶⁰Co γ照射，24 h后处死动物，取双侧股骨，行骨髓涂片和姬姆萨染色，计数含微核嗜多染红细胞（polychromatic erythrocyte，PCE）数。结果 中、高剂量组TP（25、50 μg/ml）可显著降低⁶⁰Co γ诱发的CHL染色体畸变率，S9不影响CHL细胞染色体畸变形式和畸变率。高、中剂量TP保护组（725、145 mg/kg）小鼠PCE微核率与辐照模型组相比较，相差显著（P<0.05）。结论 茶多酚能部分对抗⁶⁰Co γ辐射诱发的CHL细胞染色体畸变和微核形成，对染色体损伤具有保护作用。

Effect of tea polyphenols on chromosomal aberration of CHL cells and micronucleus formation induced by radiation

Objective To study the effect of tea polyphenols(TP) on chromosomal aberration of Chinese hamster fibroblast cells(CHL) and micronucleus formation induced by radiation.Methods CHL cells (with and without S9)were divided into control group, positive control group, radiation group and TP protective group which were given TP12.5、25、50 μg/ml. Both cells were exposed to 2 Gy ⁶⁰Co γ rays except control group and positive control group. After cultured in 1640 for 24 hours, cells were harvested for the preparation of chromosome. Subsequently, the ratio and the type of chromosomal aberration were examined. 50 mice were divided into control group, ⁶⁰Co γ ray radiation group and TP protective group which were given a gavage of TP 725, 145, 29 mg/kg. 10 mice each group. After 14 days the mice were received 6 Gy ⁶⁰Co γ radiation and were executed after radiation exposure 24 h. By means of bone marrow smear and Giemsa staining, the number of micronucleus cells per 1 000 polychromatic erythrocyte (PCE) was counted.Results The ratios of chromosomal aberration of TP protective group(25、50 μg/ml) were significantly decreased compared with radiation group. S9 has no effect on the type and rate of chromosomal aberration. Compared with radiation group, the micronucleus ratio of TP protective group (725 mg/kg and 145 mg/kg) were significantly decreased(P<0.05).Conclusion TP could partly resist chromosomal aberration of CHL cells and micronucleus formation induced by radiation. TP has protective effects on damage of chromosome.