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| 生物材料聚酰胺-胺树状大分子合成及其体外细胞相容性评价 | |
| 引用本文： | 赵光,叶玲,张瑾,王喆. 生物材料聚酰胺-胺树状大分子合成及其体外细胞相容性评价[J]. 中国生物医学工程学报, 2005, 24(6): 750-754 |
| 作者姓名： | 赵光 叶玲 张瑾 王喆 |
| 作者单位： | 1. 首都医科大学化学生物学与药学院化学生物学系,北京,100069 2. 首都医科大学基础医学院细胞生物系,北京,100069 |
| 基金项目： | 北京市科技新星计划（954812000）;北京市教委科技发展计划资助（00KJ-107）. |
| 摘 要： | 主要通过Michael加成和酰胺化逐步迭代合成反应得到聚酰胺-胺（PAMAM）树状大分子并对合成产物进行了结构表征。对生物医学有潜在应用价值的第五代（G5．0）提出了廉价易行的合成提纯步骤并通过细胞计数、MTF检测和FCM对L-929细胞系进行了体外生物相容性评价分析。结果表明，按照本试验的合成及纯化所得PAMAM G5．0对L-929细胞系在给药范围内无细胞毒性，无致瘤性，有良好的细胞相容性。 |
| 关 键 词： | 聚酰胺-胺（PAMAM） 合成 树状分子 生物相容性 |
| 文章编号： | 0258-8021(2005)06-0750-05 |
| 收稿时间： | 2004-11-08 |
| 修稿时间： | 2005-10-25 |
Synthesis of Biomaterial Polyamidoamine (PAMAM) Dendrimers and Preliminary Biological Evaluation of Biocompatibility in vitro |
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| ZHAO Guang,YE Ling,ZHANG Jin-Feng,WANG Zhe. Synthesis of Biomaterial Polyamidoamine (PAMAM) Dendrimers and Preliminary Biological Evaluation of Biocompatibility in vitro[J]. Chinese Journal of Biomedical Engineering, 2005, 24(6): 750-754 | |
| Authors: | ZHAO Guang YE Ling ZHANG Jin-Feng WANG Zhe |
| Affiliation: | 1. Department of Chemical Biology, College of Chemical Biology and Pharmacology ,Beijing 100069;2 Department of Cell Biology, College of Basic Medicine. Capital University and Medical Sciences, Beijing 100069 |
| Abstract: | A series of polyamidoamine(PAMAM) dendrimers are synthesized with an ethylenediamine core through repetitive Michael addition and amidation,which are characterized by FT-IR,NMR,etc.The economical means and process of synthesis and purification have been presented.The cellular compatibility of Generation5.0(G5.0;MW=28,788;128amines)PAMAM has been evaluated in L-929 cells by cell counting,MTT assay and flow cytometer(FCM).The data obtained clearly indicated our prepared PAMAM G5.0 have good cytocompatibility without cytotoxic and tumorigenicity. |
| Keywords: | polyamidoamine(PAMAM) synthesis dendrimers cellular compatibility |
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