PENA方法的建立及与ELISA IgM检测CMV的比较

目的　介绍一种敏感、稳定、快速、简便的实验室检测CMV的方法 ,同时探讨该种新方法与ELISA检测CMV方法的优、缺点。方法　对 5 5 2例病人应用间接荧光免疫法测定细胞核中的特异病毒早期抗体 (PENA)和ELISA法测定IgM抗体。结果　PENA方法 :强阳性 88例 ,阳性率 15 4 9% ,弱阳性 2 73例 ,阳性率 5 9 4 6 % ;ELISA -IgM方法 :阳性 34例 ,阳性率6 16 %。结论　PENA方法操作简便 ,与ELISA方法相比较 ,可对CMV感染进行早期测定及诊断 ,并可区分既往感染和即时感染 ,具有敏感性和稳定性 ,是测定小儿CMV感染的一种较好的方法

Establish the PENA method and compare with that of detecting IgM antibody Of CMV by using ELISA

A sensitive,stable,rapid and simple way of detecting cytomegalovirus(CMV)by using indirect immunofluorescence technology was introduced in this article,and to investigate the strong and weak points of two methods.Metheds: Indirect immunofluorescence technology detecting special early antibody of virus in cell nucleus and examine IgM antibody of CMV by using ELISA are used for 498 patients.Results: The methods PENA: strong positive-88 cases, rate 15.49%, weak positive-273 cases, rate-59.46%; the technology of IgM antibody of CMV by using ELISA: positive-34 cases, rate-6.16%. Conclusions: Methods of PENA is simple,sensitive and stable. It showed that the effect of detecting was early and was a good method of examing children's cytomegalovirus infection.