促结缔组织增生型成釉细胞瘤细胞的体外培养与鉴定

目的：观察体外培养的促结缔组织增生型成釉细胞瘤细胞的生长特点。方法：对促结缔组织增生型成釉细胞瘤细胞用酶消化法进行体外培养，免疫组化证实其细胞来源，以倒置显微镜观察细胞形态及生长情况．并用流式细胞仪分析细胞周期，MTT法绘制细胞生长曲线。结果：促结缔组织增生型成釉细胞瘤为上皮来源，细胞在体外培养过程中包含2种形态的细胞，与实性成釉细胞瘤细胞在体外培养相比，存在较多梭形细胞，细胞生长缓慢，培养的各代细胞未见异倍体。结论：促结缔组织增生型成釉细胞瘤细胞在体外培养过程中包含较多的梭形细胞．可能与其较多的细胞间质有关。

Cell culture and characterization of desmoplastic ameloblastoma

PURPOSE： To observe the biological behaviour of desmoplastic ameloblastoma cells in cell culture. METHODS： The specimen of desmoplastic ameloblastoma were digested with collagenase and cultured in vitro. The cell morphology was observed under inverted microscope. The expressions of cytokeratin and vimentin were detected by immunohistochemistry.The MTT assay and flow cytometry were employed to study the cell growth and proliferation. RESULTS： The characteristics of cultured desmoplastic ameloblastoma cells were of epithelial origin .They were predominantly consisted of two main kinds of cells： one was polygonal cells and the other was fusiform shape. The desmoplastic ameloblastoma cells were different from solid ameloblastoma cells in vitro. Heteroploidy type could not be found in desmoplastic ameloblastoma specimen. CONCLUSION： Desmoplastic ameloblastoma cells including more fusiform shape cells might be correlated with the mesenchyma of desmoplastic ameloblastoma cells. Supported by Shanghai Leading Academic Discipline Project （Grant No.Y0203） and Scientific Research Foundation for the Returned Oversea Chinese Scholars, Ministry of Education （Grant No.2004-176-03） and Scientific Research Foundation of Shanghai Research Institute of Stomatology （Grant No.2003-02）.