维生素E干预对静脉补铁慢性肾脏病大鼠氧化应激的影响

目的观察维生素E干预对静脉补铁慢性肾脏病(CKD)大鼠氧化应激的影响,为CKD静脉补铁治疗时进行营养干预,改善机体氧化应激状态提供实验依据。方法采用腺嘌呤灌胃方法建立大鼠CKD模型后,给予一次性尾静脉注射铁剂,同时给予维生素E干预2周,观察血浆GSH、维生素E、NO、MDA含量的变化。结果肾病对照组与正常对照组血浆GSH、维生素E、MDA均有显著性差异(P<0.05);肾病补铁组血浆GSH、维生素E低于正常对照组,血浆MDA高于肾病对照组(P<0.05);肾病补铁维生素E干预组与肾病补铁组血浆GSH、维生素E、NO、MDA均有显著性差异(P<0.05)。结论维生素E干预可提高CKD静脉补铁大鼠抗氧化能力,对缓解其体内氧化应激有重要作用。

The effects of vitamin E supplementation on oxidative stress in rats with adenine-induced kidney disease receiving intravenous iron therapy

Objective To observe the effects of vitamin E supplementation on oxidative stress in rats with chronic kidney disease(CKD) treated with intravenous iron,so as to provide an experimental basis in for guiding nutrition nursing. Methods The model of rats with CKD was induced by adenine. Then the rats with CKD were treated with a single intravenous infusion of iron and with vitamin E supplementation for two weeks. The levels of the concentration of plasma Glutathione (GSH),vitamin E (VE),nitricoxide (NO),and malondialdehyde (MDA) were measured at the end of the observation. Results There were significantl differences in plasma GSH,VE,MDA concentration between CTL group and CKD group (P<0.05). The plasma GSH and VE concentration of CKD Fe group were significantly lower than those of CTL group. And compared with CKD group,CKD Fe group exhibited a marked rise in the plasma MDA concentration (P<0.05). The differences of plasma GSH?VE?NO?MDA concentration between CKD Fe group and CKD Fe VE group were remarkable (P<0.05). Conclusion Vitamin E supplementation can enhance anti-oxidative capacity,and attenuate oxidative stress on CKD rats with a single intravenous iron therapy.