| 应用HPLC／ELSD法测定西洋参中拟人参皂苷F11的含量 |
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| 引用本文： | 鲁静,汤俊. 应用HPLC／ELSD法测定西洋参中拟人参皂苷F11的含量[J]. 药物分析杂志, 1999, 19(4): 241-243 |
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| 作者姓名： | 鲁静 汤俊 |
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| 作者单位： | 武汉市药品检验所(鲁静)，中国药品生物制品检定所(汤俊) |
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| 摘 要： | 目的：测定西洋参中拟人参皂苷Ｆ１１的含量。方法：应用高效液相色谱法－蒸发光散射检测器（ＨＰＬＣ／ＥＬＳＤ），ＰｌａｔｉｎｕｍＣ１８色谱柱，乙腈－水（３０∶７０）为流动相，并优化选择了蒸发光散射检测器参数，测定了西洋参中拟人参皂苷Ｆ１１的含量。结果：拟人参皂苷Ｆ１１在２１９２～１０９６μｇ范围内呈良好线性（ｒ＝０９９９２，ｎ＝５），回收率为（１０２６±４１）％。结论：本方法精密度、重现性良好，适用于人参皂苷类成分的分析测定。
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| 关 键 词： | 西洋参 拟人参皂苷F11 ELSD |
Application of HPLC/ELSD on Quantitative Determination ofPseudoginsenoside F11 in Radix Panacis Quinquefolii |
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| Tang Jun. Application of HPLC/ELSD on Quantitative Determination ofPseudoginsenoside F11 in Radix Panacis Quinquefolii[J]. Chinese Journal of Pharmaceutical Analysis, 1999, 19(4): 241-243 |
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| Authors: | Tang Jun |
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| Abstract: | Objective: To establish the determination method of Pseudoginsenoside F11 in Radix Panacis Quinquefolii Method: A HPLC method using evaporative light scattering detection has been developed to determine Pseudoginsenoside F11 in Radix Panacis Quinquefolii on platinum C18 column using Acetonitrile-water(3070) as mobile phase Meanwhile, the optimum conditions for the detector working and the chromatographic separation were established Result: Linear range of Pseudoginsenoside F11 was 21921096 g (r=09992,n=5), the mean recovery was (102641)% Conclusion: the precision and repeatability of this method are good respectively, and this method can be applied to the analysis of ginsenoside components |
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| Keywords: | Radix Panacis Quinquefolii Paeudoginsenoside F11 HPLC/ELSD |
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