| 分子排阻色谱法测定螺旋藻多糖纯度和分子质量 |
| |
| 引用本文： | 张旸,尹鸿萍,王旻,乐晓桐.分子排阻色谱法测定螺旋藻多糖纯度和分子质量[J].药物生物技术,2004,11(3):161-164. |
| |
| 作者姓名： | 张旸 尹鸿萍 王旻 乐晓桐 |
| |
| 作者单位： | 中国药科大学生物制药教研室,江苏,南京,210009 |
| |
| 摘 要： | 使用分子排阻色谱法，用已知平均分子质量的硫酸化葡聚糖作为标准，分别用5种不同的流动相，在Shodex Sugar KS柱上测定螺旋藻多糖的纯度以及分子质量，使用线性回归得出校正曲线。并且通过对结果进行多种方法学的考查，选取最佳的分析条件。
|
| 关 键 词： | 螺旋藻多糖 分子排阻色谱 分子质量 方法学考查 |
| 文章编号： | 1005-8915(2004)03-0161-04 |
Determination of Purity and Molecular Weight of Spinulina Platensis Polysaccharide by High Performance Gel Permeation Chromatography |
| |
| ZHANG Yang,YIN Hong-ping,WANG Min,LE Xiao-tong.Determination of Purity and Molecular Weight of Spinulina Platensis Polysaccharide by High Performance Gel Permeation Chromatography[J].Pharmaceutical Biotechnology,2004,11(3):161-164. |
| |
| Authors: | ZHANG Yang YIN Hong-ping WANG Min LE Xiao-tong |
| |
| Abstract: | Spirulina Platensis polysaccharide(PSP) was prepared in our laboratory. Its purity and molecular weight were determined by high performance gel permeation chromatography (HPGPC). The HPGPC system consisied of a two-column set,Shodex Sugar KS-804 and KS-802 columns. The M_(W) of sample was determined from the standard curves which were made by standard Dextran Sulfates. We choosed the best condition by the determination of content to the HPGPC results of PSP in five different mobile phases. |
| |
| Keywords: | Spirulina Platensis Polysaccharide HPGPC Molecular Weight Determination of content |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
