CpG寡脱氧核苷酸联合IL-2刺激慢性淋巴细胞白血病细胞的细胞遗传学研究

本研究探讨磷酸胞苷酰寡脱氧核苷酸(CpG-ODN)和白介素-2(IL-2)联合刺激培养慢性淋巴细胞白血病(CLL)细胞的遗传学特征。对115例CLL患者的外周血(或骨髓)标本,应用CpG-ODN DSP30联合IL-2刺激培养CLL细胞72小时,常规收获细胞制备染色体并进行R显带分析,探讨CLL的染色体核型特征。结果表明:115例CLL患者中,染色体分析成功率(可分析分裂相≥20个)为74.8%,异常核型检出率为58.1%。检测出一种染色体异常21例(24.4%),2种染色体异常6例(7.0%),复杂核型23例(26.7%),其中高度复杂核型9例(10.5%)。在86例患者中共检出102种163个异常,其中数目异常116个(71.2%),结构异常47个(28.8%),最常见的染色体数目异常是+12(14.0%),结构异常是15q+(5.8%)。结论:大多数CLL患者具有细胞遗传学异常,其中染色体数目异常明显多于结构异常。采用DSP30联合IL-2可以有效的增加CLL细胞的分裂相,提高CLL细胞染色体异常检出率。

Cytogenetics of Chronic Lymphocytic Leukemia Stimulated by CpG-oligodeoxynucleotids and IL-2

This study was to explore the stimulating effect of CpG-oligodeoxynucleotides（CpG-ODN） in combination with interleukin-2（IL-2） on cytogenetic features of chronic lymphocytic leukemia（CLL） cells.Peripheral blood or bone marrow cells of 115 patients with CLL were cultured for 72 hours with CpG-ODN plus interleukin-2（IL-2）,and routine karyotype analysis was performed with R-banding technique.The metaphase number ≥20 was considered as successful stimulation.The results showed that among the 115 CLL patients,successful stimulation rate was 74.8%.The rate of chromosome aberrations was 58.1%.One kind of aberration was detected in 21 cases（24.4%）,two kinds of aberration in 6 cases（7.0%）,complex aberrant karyotype in 23 cases（26.7%）,included highly complex aberrant karyotype in 9 cases（10.5%）,respectively.A total of 163 abnormalities of 102 kinds were detected in 86 patients.Number aberrations were 116（71.2%）,and structural abnormalities were 47（28.8%）.The most frequent number aberration was trisomy 12（14.0%）,and structural aberration was 15q＋（5.8%）.It is concluded that most of CLL patients have chromosome abnormality,and the number abnormality are more frequent than the structural aberrations.CpG-ODN plus IL-2 can effectively raise the number of cells at metaphase and the detection rate of chromosome aberrations in CLL patients.