Junctional and extrajunctional acetylcholine receptors in normal and denervated frog muscle fibres |
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Authors: | F Dreyer Chr Walther K Peper |
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Institution: | 1. II. Physiologisches Institut der Universit?t des Saarlandes, D-6650, Homburg/Saar, Federal Republic of Germany
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Abstract: | Summary Ionic channel properties of acetylcholine receptors located in, in the vicinity of, or far away from a frog neuromuscular
junction were investigated by noise analysis of drug induced current fluctuations. For drugs applied to the junction, in certain
cases two Lorentzian curves were necessary to describe the data. It is postulated that the reason for this observation is
that a contribution from perijunctional receptors was being observed. The conductance of a single channel in the junction
was independent of the nature of the agonist and had an average value of 17.9 pS (temperature range 8–25°C, solution buffered
with Tris). After denervation for 21 days the conductance γ was 7.5 pS at extrajunctional locations. In the close neighbourhood
of the junction (perijunctional receptors) values were found between 4 and 19 pS.
The mean value of the open channel life-time τ in the endplate exposed to acetylcholine was 2.4 ms at 8–11°C. This value was
0.90 ms with carbachol, 0.50 ms with succinylcholine, 0.28 ms with decamethonium and 0.45 ms with nicotine. The receptors
outside the endplate exhibited τ-values which at a given temperature were 2–3 times larger than those at the endplate. Raising
the temperature to 23°C reduced all τ-values by factors of 2–3. It is concluded that at least two types of ACh-receptors with
different properties exist in the muscle membrane, possibly produced by ACh-receptive units in different states of aggregation.
This work was supported by the Deutsche Forschungsgemeinschaft, SFB 38, Project N |
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Keywords: | Neuromuscular junction Noise analysis Denervation Drugs Cholinesterase inhibitors Frogs |
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