| RNA干扰下调p53抑制因子iASPP表达对膀胱癌细胞的影响 |
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| 引用本文： | 刘涛,李琳,贾麾,荆宏伟,孔垂泽. RNA干扰下调p53抑制因子iASPP表达对膀胱癌细胞的影响[J]. 中华泌尿外科杂志, 2011, 32(1): 239-243. DOI: 10.3760/cma.j.issn.1000-6702.2011.04.007 |
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| 作者姓名： | 刘涛 李琳 贾麾 荆宏伟 孔垂泽 |
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| 作者单位： | 中国医科大学附属第一医院泌尿外科,沈阳,110001;广东省人民医院康复科;中国医科大学; |
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| 基金项目： | 辽宁省博士科研启动基金 |
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| 摘 要： | Objective To discuss the effects of silencing of iASPP gene on human bladder cancer cells. Methods RNAi silencing of iASPP gene in bladder cancer cell 5637 and T24 cells were used by lentiviral mediated interfering short hairpin RNAs. Cell proliferation was tested by MTT assay, and rate of colony was tested by colony formation assay. Cell cycles were tested by using fluorescence-activated cell sorting. Results Down-regulation of iASPP could inhibit the growth and proliferation of human bladder cancer cells (P＜0.05). iASPP know-down could decrease the colony formation of 5637 and T24 cells (P＜0, 05). Knocking down of iASPP in 5637 and T24 cells showed cell arrested at G1. Conclusions Silencing of iASPP gene could inhibit proliferation and colony formation of bladder cancer, iASPP might be an important target for gene therapy of bladder cancer.
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| 关 键 词： | RNA干扰 肿瘤抑制蛋白质p53 膀胱肿瘤 癌 |
Effects of silencing of iASPP gene on human bladder cancer cells |
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| LIU Tao,LI Lin,JIA Hui,JING Hong-wei,KONG Chui-ze. Effects of silencing of iASPP gene on human bladder cancer cells[J]. Chinese Journal of Urology, 2011, 32(1): 239-243. DOI: 10.3760/cma.j.issn.1000-6702.2011.04.007 |
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| Authors: | LIU Tao LI Lin JIA Hui JING Hong-wei KONG Chui-ze |
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| Abstract: | |
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| Keywords: | iASPPRNA interferenceiASPPTumor suppressor protein p53Urinary bladder neoplasmsCarcinoma |
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