| 流体剪切力作用强度对极化破骨细胞ATP6V1a1 mRNA的影响 |
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| 引用本文： | 张庆鸿,梁星,刘梦桃,朱保民,付俊. 流体剪切力作用强度对极化破骨细胞ATP6V1a1 mRNA的影响[J]. 华西口腔医学杂志, 2007, 25(4): 396-398 |
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| 作者姓名： | 张庆鸿 梁星 刘梦桃 朱保民 付俊 |
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| 作者单位： | 浙江大学医学院附属口腔医院,修复科,浙江,杭州,310006;口腔生物医学工程教育部重点实验室,四川大学,四川,成都,610041;口腔生物医学工程教育部重点实验室,四川大学,四川,成都,610041;云南省昆明红十字会医院,修复科,云南,昆明,650021 |
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| 摘 要： | 目的观察不同作用强度流体剪切力对鼠极化破骨细胞ATP6V1a1 mRNA表达水平的影响。方法联合应用形态学观察、特异性染色和吸收实验，对长骨机械分离法获得的正在进行骨吸收的极化破骨细胞进行培养鉴定。然后将细胞分为对照组和0.9、2.9、8.7、26.3 dynes/cm2实验组，作用时间30 min，行实时荧光定量PCR检测ATP6V1a1 mRNA表达水平，并进行统计分析。结果获得的细胞满足破骨细胞的鉴定标准，属于正在进行骨吸收的极化破骨细胞。对照组和0.9、2.9、8.7、26.3 dynes/cm2实验组ATP6V1a1 mRNA表达量分别为（1.14±0.06）×106、（1.62±0.09）×106、（2.28±0.13）×106、（3.24±0.18）×106、（9.16±0.53）×106 个拷贝数，所有组间均有显著性差异（P<0.05）。结论极化破骨细胞对流体剪切力反应敏感，随着加载强度的增加，ATP6V1a1 mRNA表达水平有增加趋势。
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| 关 键 词： | 破骨细胞 流体剪切力 骨吸收 |
| 文章编号： | 1000-1182（2007）04-0396-03 |
| 收稿时间： | 2007-08-25 |
| 修稿时间： | 2006-11-04 |
Effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in polarized osteoclasts |
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| ZHANG Qing-hong,LIANG Xing,LIU Meng-tao,ZHU Bao-min,FU Jun. Effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in polarized osteoclasts[J]. West China journal of stomatology, 2007, 25(4): 396-398 |
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| Authors: | ZHANG Qing-hong LIANG Xing LIU Meng-tao ZHU Bao-min FU Jun |
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| Affiliation: | 1. Dept. of Prosthodontics, The Affiliated Stomatology Hospital of Medical College, Zhejiang University, Hangzhou 310006, China; 2. Key. Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China；3. Dept. of Prosthodontics, The Red Cross Hospital of Kunming, Kunming 650021, China |
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| Abstract: | OBJECTIVE: To observe effects of fluid shear stress strength on mRNA expression of ATP6V1a1 in rat polarized osteoclasts. METHODS: Rat polarized osteoclasts suffered 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 fluid shear stress for 30 min. mRNA expression of ATP6V1a1 was detected by Real-Time fluorescent quantitation PCR. RESULTS: mRNA expression of ATP6V1a1 in 0.0 (control group), 0.9, 2.9, 8.7 and 26.3 dynes/cm2 groups was (1.14 +/- 0.06) x 10(6), (1.62 +/- 0.09) x 10(6), (2.28 +/- 0.13) x 10(6), (3.24 +/- 0.18) x 10(6), (9.16 +/- 0.53) x 10(6) copy numbers, respectively (P < 0.05). CONCLUSION: In the present study, polarized osteoclasts are sensitive to fluid shear stress. mRNA expression of ATP6V1a1 has increscent tendency along with increasing of fluid shear stress strength. |
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| Keywords: | osteoclast fluid shear stress strength bone resorption |
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