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| 氯离子通道CIC-5在大鼠牙胚发育过程中的表达 | |
| 引用本文： | 侯晋 段小红 司徒镇强. 氯离子通道CIC-5在大鼠牙胚发育过程中的表达[J]. 华西口腔医学杂志, 2007, 25(5): 444-446,453 |
| 作者姓名： | 侯晋 段小红 司徒镇强 |
| 作者单位： | 第四军医大学口腔医学院口腔生物学教研室,陕西西安710032 |
| 基金项目： | 国家自然科学基金资助项目（371540） |
| 摘 要： | 目的研究氯离子通道ClC-5在大鼠牙胚发育过程中的表达情况，探讨其在牙齿发育过程中的作用。方法从不同发育阶段的大鼠牙胚中提取总RNA和蛋白，应用RT—PCR和Westem blot检测ClC-5在大鼠牙胚发育过程中mRNA和蛋白的表达情况。结果ClC-5在牙胚发育的过程中存在时空特异性表达，其mRNA和蛋白都主要在牙胚发育的钟状晚期表达。结论ClC-5在大鼠牙胚发育过程中的这种时空特异性表达，提示其可能在牙齿发育的过程中发挥一定的作用。 |
| 关 键 词： | 氯离子通道 牙胚 发育 |
| 文章编号： | 1000-1182（2007）05-0444-03 |
| 修稿时间： | 2006-10-192007-02-01 |
Expression of CIC-5 in rat tooth germ development |
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| HOU Jin, DUAN Xiao-hong, SI-TU Zhen-qiang.. Expression of CIC-5 in rat tooth germ development[J]. West China journal of stomatology, 2007, 25(5): 444-446,453 | |
| Authors: | HOU Jin DUAN Xiao-hong SI-TU Zhen-qiang. |
| Affiliation: | Department of Oral Biology, School of Stomatology, The Fourth Military Medical University, Xi'an, China. |
| Abstract: | Objective To determine the expression and possible function of CIC-5 during the rat tooth germ development. Methods Isolated total mRNA and protein from the rat tooth germs which were at different stages of development. RT-PCR and Western blot were used to investigate the mRNA and protein expression of the ClC-5 in the rat tooth germ. Results The mRNA and protein of ClC-5 expressed in the late bell stage, but undetected in the early or middle bell stage. Conclusion These results showed the spatial temporal distribution of expression of ClC-5 during the different stages of the rat tooth germ development and suggested that it might contribute to the rat tooth germ development. |
| Keywords: | chloride channel tooth germ development |
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