原核表达重组人骨形态发生蛋白-7对牙槽骨修复再生的实验研究

目的研究原核表达重组人骨形态发生蛋白- 7（rhBMP- 7）对牙槽骨修复再生的影响。方法采用新西兰白兔建立动物模型，以明胶海绵作为载体，将大肠杆菌原核表达的rhBMP- 7与之复合后植入即刻拔除牙齿的牙槽窝内进行干预治疗，术后2、4、8及12周处死动物，取标本进行大体观察、扫描电镜分析、碱性磷酸酶（ALP）活性测定。结果大体观察结果显示，实验组和对照组牙槽嵴高度的吸收差异有显著性；扫描电镜观测显示，实验组的骨创愈合比对照组大约提前4～6周；ALP活性测定显示实验组均明显高于对照组，其差异有统计学意义（P<0.05）。结论原核表达rhBMP- 7具有促进新骨修复再生、防止牙槽骨吸收的作用。

Effect of recombinant human bone morphogenetic protein-7 expressed in prokaryocyte to augment bone formation of alveolar ridge

Objective To investigate new bone formation of alveolar augmentation with recombinant human bone morphogenetic protein-7(rhBMP-7) expressed in prokaryocyte.Methods To create the model of rabbit extraction socket into which the composites of rhBMP-7 and the gelatin sponge was immediately implanted,then the samples were investigated 2,4,8 and 12 weeks postoperatively by gross observation,scanning electron microscope(SEM),quantitative measurement of calcium content and alkaline phosphatase(ALP) activity.Results There was significant difference in height of alveolar ridge absorpted between the experimental groups and the carrier control groups through gross observation.The result of SEM showed that bone healing in rhBMP-7 groups was 4-6 weeks earlier than that of control groups.ALP activity in rhBMP-7 groups were obviously high compared with that of control groups.Conclusion The BMP-7 has a satisfactory osteoinduction ability to promote new bone formation and prevent alveolar bone absorption.