| 口腔癌相关成纤维细胞对舌癌细胞株细胞外信号调节激酶通路的影响 |
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| 引用本文： | 林靖雯,陈谦明,李胜富,付春华,林梅,龙丹,周红梅. 口腔癌相关成纤维细胞对舌癌细胞株细胞外信号调节激酶通路的影响[J]. 华西口腔医学杂志, 2007, 25(5): 490-492 |
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| 作者姓名： | 林靖雯 陈谦明 李胜富 付春华 林梅 龙丹 周红梅 |
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| 作者单位： | 口腔生物医学工程教育部重点实验室,四川大学;四川大学华西口腔医院,黏膜科;四川大学华西医院,卫生部移植工程和移植免疫重点实验室,四川,成都,610041 |
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| 基金项目： | 国家自然科学基金 , 四川省科技攻关项目 |
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| 摘 要： | 目的研究口腔癌相关成纤维细胞（CAFs）对舌癌细胞株细胞外信号调节激酶（ERK）通路的影响。方法以口腔CAFs条件培养基刺激舌癌细胞株Tca8113和将Tca8113细胞与口腔CAFs共同培养，采用Western杂交检测特定时间Tca8113细胞总ERK和总pERK的表达。结果Tca8113细胞经口腔CAFs条件培养基刺激或与口腔CAFs共同培养后，总pERK的表达迅速增加，总pERK与总ERK的比值增加。结论口腔CAFs对癌细胞ERK通路有活化作用。
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| 关 键 词： | 癌相关成纤维细胞 舌癌 细胞外信号调节激酶传导通路 |
| 文章编号： | 1000-1182（2007）05-0490-03 |
| 收稿时间： | 2007-10-25 |
| 修稿时间： | 2006-10-08 |
Effect of oral carcinoma-associated fibroblasts on extracellular signal-regulated kinases pathway in the lingual carcinoma cell line |
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| LIN Jing-wen,CHEN Qian-ming,LI Sheng-fu,FU Chun-hua,LIN Mei,LONG Dan,ZHOU Hong-mei. Effect of oral carcinoma-associated fibroblasts on extracellular signal-regulated kinases pathway in the lingual carcinoma cell line[J]. West China journal of stomatology, 2007, 25(5): 490-492 |
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| Authors: | LIN Jing-wen CHEN Qian-ming LI Sheng-fu FU Chun-hua LIN Mei LONG Dan ZHOU Hong-mei |
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| Affiliation: | 1. Key. Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China；2. Dept. of Oral Medicine, West China College of Stomatology, Sichuan University, Chengdu 610041, China; 3. Key. Laboratory of Transplantation Engineering and Transplantation Immunity of Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China |
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| Abstract: | Objective To investigate the effects of oral carcinoma-associated fibroblasts(CAFs) on extracellular signal-regulated kinases(ERK) pathway in a lingual carcinoma cell line. Methods The lingual carcinoma cell line, Tca8113 was stimulated by conditioned medium from oral CAFs, or cocultured with oral CAFs for definite time. Total ERK and pERK in Tca8113 lysate were detected by Western blotting, and the ratio between pERK and ERK were calculated. Results Both stimulation by conditioned medium and coculture induced prompt phosphorylation of ERK, and increased the ratio between pERK and ERK. Conclusion Oral CAFs can activate ERK pathway of carcinoma cells. |
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| Keywords: | carcinoma-associated fibroblasts tongue carcinoma extracellular signal-regulated kinases pathway |
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