|
|||||
|
|
| 异鸟苷掺入的富鸟嘌呤寡核苷酸的合成 | |
| 引用本文： | 王延龄,潘鹤龄,杨振军,张亮仁,张礼和. 异鸟苷掺入的富鸟嘌呤寡核苷酸的合成[J]. 中国药物化学杂志, 2004, 14(4): 225-228 |
| 作者姓名： | 王延龄 潘鹤龄 杨振军 张亮仁 张礼和 |
| 作者单位： | 北京大学药学院天然药物及仿生药物国家重点实验室,北京,100083 |
| 基金项目： | 国家自然科学基金，教育部重点实验室基金 |
| 摘 要： | 目的改进异鸟苷单体的合成路线,合成异鸟苷掺入的富鸟嘌呤的寡核苷酸.方法以6-O-苄基鸟嘌呤为原料,经环氧开环、催化氢化、水解还原制备异鸟苷,经多步反应后得到异鸟苷亚磷酰化产物,采用固相合成方法合成异鸟苷掺入的富含鸟嘌呤的寡核苷酸.结果与结论经环氧开环能够区域选择性地得到N9取代的单一产物,收率提高到40%,并成功地合成了异鸟苷掺入的富含嘌呤十六聚寡核苷酸.本合成路线具有区域选择性强、路线简单、易操作、收率高等优点. |
| 关 键 词： | 药物化学 制备 化学合成 寡核苷酸 异鸟苷 区域选择性 |
| 文章编号： | 1005-0108(2004)04-0225-04 |
| 修稿时间： | 2004-03-06 |
Synthesis of iso-guanosine incorporated G-rich oligonucleotide |
|
| WANG Yan-ling,PAN He-ling,YANG Zhen-jun,ZHANG Liang-ren,ZHANG Li-he. Synthesis of iso-guanosine incorporated G-rich oligonucleotide[J]. Chinese Journal of Medicinal Chemistry, 2004, 14(4): 225-228 | |
| Authors: | WANG Yan-ling PAN He-ling YANG Zhen-jun ZHANG Liang-ren ZHANG Li-he |
| Abstract: | Aim To improve the synthesis of isoguanosine monomer and to synthesize isoguanosine incorporated G-rich oligonucleotide.Methods Starting from 6- O -benzylguanine which reacted with epoxide 1,then was hydrolyzed and reduced,the isoguanosine was achieved.The phosphoroimidated isoguanosine was then given via several steps and solid-phase synthesis strategy was used for the synthesis of G-rich oligonucleotide.Results N9 subsititued isoguanosine was obtained regioselectively by the ring open reaction,and the yield was improved to 40%.The isoguanosine incorporated 16-mer G-rich oligonucleotide was successfully synthesized. |
| Keywords: | medicinal chemistry preparation chemical synthesis oligonucleotide isoguanosine regioselectivity |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |