针刺对神经干细胞海马内移植痴呆大鼠学习记忆和海马胆碱能系统的影响

目的:观察针刺对神经干细胞(NSCs)海马内移植老年性痴呆(AD)大鼠学习记忆和海马胆碱能系统的影响,探讨针刺治疗老年性痴呆的机制。方法:采用β-淀粉样蛋白(β-AP)向海马CA1区定向注射制做AD模型,将大鼠分为五组:正常组、模型组、移植组、针刺组和移植针刺组,每组各12只。针刺选取百会、大椎、人中三个穴位,并接通电针仪。大鼠跳台检测行为学指标;酶免法检测海马组织胆碱乙酰转移酶(ChAT)、乙酰胆碱酯酶(AchE)含量。结果:移植针刺组大鼠跳台潜伏期比模型组增加(P<0.05),错误次数比模型组减少(P<0.05);海马组织ChAT含量比模型组提高(P<0.05),AchE含量比模型组降低(P<0.05);移植针刺组与移植组比较有显著性差异(P<0.05)。结论:针刺对大鼠海马胆碱能系统的改善是治疗AD的作用机制之一,针刺结合神经干细胞移植治疗效果更佳。

Observing the Effect of Acupuncture on Metabolism of Study Memorizing Ability and the Quantity of Ache System in Hippocampus of AD Rats

Objective ： To observe the effect of acupuncture on the memorizing ability and the quantity of AchE system in hip-pocampus of AD （Alzheimer＇s Disease） rats that neural stem cell was transplanted in hippocampus, and discussing the mecha-nism of acupuncture to cure the AD. Methods ： The model was made by injectingβ-AP to CA1 of hippocampus, the rats were randomized divided into normal group, model group, transplanted group, acupunctural group and transplanted acupuncture group, each had 12 rats. The acupuncture were elected on Baihui, Dazhui and Renzhong and connected the electronic needle device. The memorizing ability was measured by using the diving tower of rats and enzyme-immunossay methods in cerebral cor-tex, the content of hippocamous ChAT and AchE were examined. Results ： Incubation period of step down of the transplanted acu-puncture group increased （ P〈0.05 ） that compared with the model group; the correct times of the transplanted acupuncture group decreased （P〈0. 05） that compared with the model group; the quantity of Ache in hippocampus of the transplanted acupuncture group decreased （P〈0. 05） that compared with the model group while the quantity of ChAT in hippecampus increased （P〈 0. 05 ） that compared with the model group; there were obvious differences between the transplanted acupuncture group and the transplanted group （P〈0.05）. Conclusion： The quantity of AchE system in brain of AD rats can be improved by acupuncture and acupuncture combined with neural stem cell transplanting will be better.