非小细胞肺癌的miRNA-29a表达及临床意义

目的 探讨miRNA-29a在非小细胞肺癌（NSCLC）中的表达及临床意义。方法 采用实时荧光定量PCR技术检测55例NSCLC组织及癌旁组织中miRNA-29a的表达水平，并分析其与临床病理特征及预后的关系。结果 NSCLC组织中miRNA-29a的表达下调43例，表达上调12例，其表达仅与性别有关（P＜0.05），而与年龄、吸烟史、病理类型、肿瘤大小及TNM分期均无关。miRNA 29a表达诊断癌组织与癌旁组织的受试者工作特征（ROC）曲线的面积（Az）为0.669（95％CI：0.568～0.770），灵敏度和特异度分别为49.1％和85.5％。生存分析显示，不同miRNA-29a表达的NSCLC患者的生存率有明显差异（P=0.032）；多因素回归分析显示，miRNA-29a表达是总生存期的独立预后因素。结论 miRNA-29a在NSCLC组织中表达下调，其可作为NSCLC的诊断、预后判断指标及治疗靶标。

Expression of miRNA-29a in non-small cell lung cancer and clinical significance

Objective To explore the expression of miRNA-29a in non-small cell lung cancer（NSCLC） and its relationship with clinical pathological features and prognosis. Methods The expressions of miRNA-29a in 55 NSCLC and adjacent cancerous tissues were tested by real-time PCR with U6 as internal control. Results NSCLC tissues were divided into 2 groups according to the expression levels of miRNA-29a, down-regulation group （n = 43 ） and up-regulation group（ n = 12）. miRNA-29a expression only correlated to gender,while didn＇t correlate with age, smoking history, histologic type, tumor size and TNM staging, miRNA-29a expression had value of diagnosis in NSCLC by ROC curve. Az of RDC curve was 0. 669 （95 % CI： 0, 568-0, 770）, and sensitivity and specificity were 49. 1% and 85.5%. There was a significant difference of overall survival down-regulation group and up-regulation group. Cox regression analysis showed expression of miRNA-29a was an independent prognosis factor of overall survival. Conclusion The expression of miRNA-29a is down-regulated in NSCLC. miRNA-29a may not only act as a signature of diagnosis and predictor of prognosis, but also as a therapeutic target in NSCLC,