肝/肾/骨型碱性磷酸酶基因ALPL抑制卵巢癌细胞系SKOV3增殖的研究

目的 研究肝/肾/骨型碱性磷酸酶基因(alkaline phosphatase,liver/bone/kidney,ALPL)在卵巢癌细胞株中的表达及其对卵巢癌细胞SKOV3增殖的影响.方法 通过qRT-PCR检测ALPL在不同卵巢癌细胞株(HEY、SKOV3、8910、ES-2、OVCAR3、3AO、A2780)mRNA中的表达,并与正常卵巢组织进行比较.在低表达ALPL的卵巢癌细胞株SKOV3中外源性过表达ALPL,通过MTT法、平板克隆实验检测细胞的增殖,流式细胞仪检测细胞周期.通过建立裸鼠皮下种植瘤模型,观察过表达ALPL的SKOV3细胞在体内的成瘤能力.结果 ALPL在卵巢癌细胞中的mRNA表达低于正常卵巢组织(P＜0.01),外源性过表达ALPL可抑制SKOV3细胞的增殖和克隆形成率(P＜0.01),阻滞细胞G1期向S的转化,并抑制肿瘤细胞的成瘤能力(P＜0.05).结论 ALPL表达的恢复可以明显抑制卵巢癌细胞SKOV3的增殖及成瘤能力.

Alkaline phosphatase,liver/bone/kidney,inhibits proliferation of ovarian cancer cell line SKOV3

Objective To investigate the expression of tissue-nonspecific alkaline phosphatase,liver/bone/kidney (ALPL) in human ovarian cancer cell lines,and determine its effect on the proliferation of ovarian cancer SKOV3 cells.Methods qRT-PCR was used to detect the expression of ALPL mRNA level in 7 ovarian cancer cell lines (HEY,SKOV3,8910,ES-2,OVCAR3,3AO and A2780).The results were compared with its level in normal ovarian tissues.After the SKOV3 cells (with low expression of ALPL) were interfered with lentiviral vector to induce ALPL overexpression,cell proliferation was measured by MTT assay and plate colony formation assay,and cell cycle were assessed by flow cytometry.Tumorigenesis was evaluated through xenograft growth of ALPL-overexpressed SKOV3 cells in nude mice.Results The expression level of ALPL was significantly lower in all the ovarian cancer cell lines studied when compared with that in normal ovarian tissues (P ＜ 0.01).ALPL Over-expression resulted in the decreases in cell proliferation and clone formation rate (P ＜ 0.01) and blocked the transition of G1 phase to S phase in SKOV3 cells,as well as suppressed the subcutaneous tumorigenesis in xenografts tumor model (P ＜ 0.05).Conclusion Reversion of ALPL expression level might obviously suppress the cell proliferation and tumorigenesis of SKOV3 cells.